In vitro display technologies: novel developments and applications

In vitro display techniques are powerful tools to select polypeptide binders against various target molecules. Novel applications include maturation of protein affinity and stability, selection for enzymatic activity, and the display of cDNA and random polypeptide libraries. Taken together, these di...

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Bibliographic Details
Published in:Current Opinion in Biotechnology 2001-08, Vol.12 (4), p.400-405
Main Authors: Amstutz, Patrick, Forrer, Patrik, Zahnd, Christian, Plückthun, Andreas
Format: Article
Language:English
Subjects:
Binding Sites - physiology
directed evolution
Directed Molecular Evolution - methods
DNA, Complementary - genetics
DNA, Complementary - metabolism
Drug Evaluation, Preclinical - methods
Enzyme Stability - physiology
Gene Library
In vitro display
maturation
mRNA display
Mutagenesis - genetics
Peptide Library
preselection
Proteins - genetics
Proteins - metabolism
proteomics
ribosome display
Ribosomes - genetics
Ribosomes - metabolism
RNA, Messenger - genetics
RNA, Messenger - metabolism
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Summary:In vitro display techniques are powerful tools to select polypeptide binders against various target molecules. Novel applications include maturation of protein affinity and stability, selection for enzymatic activity, and the display of cDNA and random polypeptide libraries. Taken together, these display techniques have great potential for biotechnological, medical and proteomic applications.
ISSN:0958-1669
1879-0429
DOI:10.1016/S0958-1669(00)00234-2