Modulatory Effect of Taurine on 7,12-Dimethylbenz(a)Anthracene-Induced Alterations in Detoxification Enzyme System, Membrane Bound Enzymes, Glycoprotein Profile and Proliferative Cell Nuclear Antigen in Rat Breast Tissue

• Published in: Journal of biochemical and molecular toxicology 2016-08, Vol.30 (8), p.414-423
• Main Authors: Vanitha, Manickam Kalappan, Baskaran, Kuppusamy, Periyasamy, Kuppusamy, Selvaraj, Sundaramoorthy, Ilakkia, Aruldoss, Saravanan, Dhiravidamani, Venkateswari, Ramachandran, Revathi Mani, Balasundaram, Anandakumar, Pandi, Sakthisekaran, Dhanapal
• Format: Article
• Language: English
• Subjects: 9,10-Dimethyl-1,2-benzanthracene - toxicity; Animals; Antineoplastic Agents - pharmacology; Breast Cancer; Carcinogens - toxicity; Cytochromes - genetics; Cytochromes - metabolism; DMBA; Drug Metabolizing Enzymes; Female; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Glycoproteins; Glycoproteins - genetics; Glycoproteins - metabolism; Mammary Glands, Animal - drug effects; Mammary Glands, Animal - metabolism; Mammary Neoplasms, Experimental - chemically induced; Mammary Neoplasms, Experimental - drug therapy; Mammary Neoplasms, Experimental - genetics; Mammary Neoplasms, Experimental - metabolism; Membrane Bound Enzymes; Membrane Transport Proteins - genetics; Membrane Transport Proteins - metabolism; Metabolic Detoxication, Phase I - genetics; Metabolic Detoxication, Phase II - genetics; Proliferating Cell Nuclear Antigen - genetics; Proliferating Cell Nuclear Antigen - metabolism; Proliferative Cell Nuclear Antigen; Protein Carbonylation; Rats; Rats, Sprague-Dawley; Taurine; Taurine - pharmacology
• ISSN: 1095-6670; 1099-0461
• DOI: 10.1002/jbt.21805

ABSTRACT The modulatory effect of taurine on 7,12‐dimethylbenz(a)anthracene (DMBA)‐induced breast cancer in rats was studied. DMBA (25 mg/kg body weight) was administered to induce breast cancer in rats. Protein carbonyl levels, activities of membrane bound enzymes (Na+/K+ATPase, Ca2+ATPase, and Mg2+ATPase), phase I drug metabolizing enzymes (cytochrome P450, cytochrome b5, NADPH cytochrome c reductase), phase II drug metabolizing enzymes (glutathione‐S‐transferase and UDP‐glucuronyl transferase), glycoprotein levels, and proliferative cell nuclear antigen (PCNA) were studied. DMBA‐induced breast tumor bearing rats showed abnormal alterations in the levels of protein carbonyls, activities of membrane bound enzymes, drug metabolizing enzymes, glycoprotein levels, and PCNA protein expression levels. Taurine treatment (100 mg/kg body weight) appreciably counteracted all the above changes induced by DMBA. Histological examination of breast tissue further supported our biochemical findings. The results of the present study clearly demonstrated the chemotherapeutic effect of taurine in DMBA‐induced breast cancer.