A combination of solid-phase extraction and dispersive solid-phase extraction effectively reduces the matrix interference in liquid chromatography-ultraviolet detection during pyraclostrobin analysis in perilla leaves

Perilla leaves contain many interfering substances; thus, it is difficult to protect the analytes during identification and integration. Furthermore, increasing the amount of sample to lower the detection limit worsens the situation. To overcome this problem, we established a new method using a comb...

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Bibliographic Details
Published in:Biomedical chromatography 2015-12, Vol.29 (12), p.1932-1936
Main Authors: Farha, Waziha, Rahman, Md. Musfiqur, Abd El-Aty, A. M., Jung, Da-I., Kabir, Md. Humayun, Choi, Jeong-Heui, Kim, Sung-Woo, Jeong Im, So, Lee, Young-Jun, Shin, Ho-Chul, Kwon, Chan-Hyeok, Son, Young-Wook, Lee, Kang-Bong, Shim, Jae-Han
Format: Article
Language:English
Subjects:
Carbamates - analysis
Chromatography, Liquid - methods
interferences
LC-UVD
Limit of Detection
Linear Models
Perilla
Perilla - chemistry
perilla leaf
Plant Leaves - chemistry
pyraclostrobin
Pyrazoles - analysis
Reproducibility of Results
Solid Phase Extraction - methods
SPE-d-SPE combination
Strobilurins
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Summary:Perilla leaves contain many interfering substances; thus, it is difficult to protect the analytes during identification and integration. Furthermore, increasing the amount of sample to lower the detection limit worsens the situation. To overcome this problem, we established a new method using a combination of solid‐phase extraction and dispersive solid‐phase extraction to analyze pyraclostrobin in perilla leaves by liquid chromatography with ultraviolet absorbance detection. The target compound was quantitated by external calibration with a good determination coefficient (R2 = 0.997). The method was validated (in triplicate) with three fortification levels, and 79.06– 89.10% of the target compound was recovered with a relative standard deviation
ISSN:0269-3879
1099-0801
DOI:10.1002/bmc.3523