Clinical application of a ligation-independent pathway of multiplex ligation-dependent probe amplification for the determination of quinolone susceptibility of Streptococcus pneumoniae

We previously uncovered a ligation-independent pathway of multiplex ligation-dependent probe amplification (MLPA) through which products of MLPA could be amplified without both hybridization and ligation reactions. Here, we utilized this pathway to detect an antibiotic resistance mutation of quinolo...

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Bibliographic Details
Published in:Journal of microbiological methods 2016-09, Vol.128, p.13-15
Main Authors: Uno, Naoki, Araki, Nobuko, Kaku, Norihito, Kosai, Kosuke, Hasegawa, Hiroo, Yanagihara, Katsunori
Format: Article
Language:English
Subjects:
DNA Gyrase - genetics
DNA Mutational Analysis
DNA, Bacterial - genetics
Drug Resistance, Bacterial
Multiplex ligation-dependent probe amplification
Multiplex Polymerase Chain Reaction
Quinolone resistance-determining regions
Quinolones - pharmacology
Streptococcus pneumoniae
Streptococcus pneumoniae - drug effects
Streptococcus pneumoniae - genetics
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Summary:We previously uncovered a ligation-independent pathway of multiplex ligation-dependent probe amplification (MLPA) through which products of MLPA could be amplified without both hybridization and ligation reactions. Here, we utilized this pathway to detect an antibiotic resistance mutation of quinolones in Streptococcus pneumoniae. •A novel assay using a ligation-independent pathway of multiplex ligation-dependent probe amplification was developed.•Quinolone-resistant clinical isolates of Streptococcus pneumoniae were successfully detected by the ligation-independent probe amplification system.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2016.06.024