A toolbox to measure changes in the cell wall glycopolymer composition during differentiation of Streptomyces coelicolor A3(2)

Cell wall glycopolymers (CWG) represent an important component of the Gram-positive cell envelope with many biological functions. The mycelial soil bacterium Streptomyces coelicolor A3(2) incorporates two distinct CWGs, polydiglycosylphosphate (PDP) and teichulosonic acid, into the cell wall of its...

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Bibliographic Details
Published in:Journal of microbiological methods 2016-09, Vol.128, p.52-57
Main Authors: Sigle, Steffen, Steblau, Nadja, Wohlleben, Wolfgang, Muth, Günther
Format: Article
Language:English
Subjects:
Cell Wall - chemistry
Colorimetric assay
Colorimetry
Galactose - chemistry
Hexosamines - chemistry
Kdn
Polydiglycosylphosphate
Spores, Bacterial - chemistry
Streptomyces coelicolor
Streptomyces coelicolor - chemistry
Streptomyces coelicolor - cytology
Teichulosonic acid
Uronic Acids - chemistry
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Summary:Cell wall glycopolymers (CWG) represent an important component of the Gram-positive cell envelope with many biological functions. The mycelial soil bacterium Streptomyces coelicolor A3(2) incorporates two distinct CWGs, polydiglycosylphosphate (PDP) and teichulosonic acid, into the cell wall of its vegetative mycelium but only little is known about their role in the complex life cycle of this microorganism. In this study we established assays to measure the total amount of CWGs in mycelial cell walls and spore walls, to quantify the individual CWGs and to determine the length of PDP. By applying these assays, we discovered that the relative amount of CWGs, especially of PDP, is reduced in spores compared to vegetative mycelium. Furthermore we found that PDP extracted from mycelial cell walls consisted of at least 19 repeating units, whereas spore walls contained substantially longer PDP polymers. •Establishment of colorimetric assays for the quantification of S. coelicolor cell wall glycopolymers•Development of a PAGE system for separation of polydiglycosylphosphate fragments•Characterization of the cell wall glycopolymer composition during differentiation of S. coelicolor A3(2)
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2016.07.002