Genetically modified bacteriophages in applied microbiology

Summary Bacteriophages represent a simple viral model of basic research with many possibilities for practical application. Due to their ability to infect and kill bacteria, their potential in the treatment of bacterial infection has been examined since their discovery. With advances in molecular bio...

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Bibliographic Details
Published in:Journal of applied microbiology 2016-09, Vol.121 (3), p.618-633
Main Authors: Bardy, P, Pantcek, R, Benesik, M, Doskar, J
Format: Article
Language:English
Subjects:
Animals
Antibiotics
Apoptosis
Bacteria
Bacteria - drug effects
Bacterial diseases
Bacterial infections
Bacterial Infections - drug therapy
bacteriophages
Bacteriophages - genetics
Biofilms
Biological Therapy
biopharmaceuticals
Biosensing Techniques
Biotechnology
Biotechnology - methods
Cell culture
Cell death
Drug delivery systems
enzybiotics
Food contamination & poisoning
Food industry
Food processing industry
Gene transfer
Genes
Genetic Engineering
Genetic modification
genetically modified bacteriophages
Genomes
Host range
Humans
Industrial Microbiology
Insertion
Lytic enzymes
Microbiology
Molecular biology
Molecular chains
pathogen detection
Pathogens
phage therapy
Phages
Viability
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Summary:Summary Bacteriophages represent a simple viral model of basic research with many possibilities for practical application. Due to their ability to infect and kill bacteria, their potential in the treatment of bacterial infection has been examined since their discovery. With advances in molecular biology and gene engineering, the phage application spectrum has been expanded to various medical and biotechnological fields. The construction of bacteriophages with an extended host range or longer viability in the mammalian bloodstream enhances their potential as an alternative to conventional antibiotic treatment. Insertion of active depolymerase genes to their genomes can enforce the biofilm disposal. They can also be engineered to transfer various compounds to the eukaryotic organisms and the bacterial culture, applicable for the vaccine, drug or gene delivery. Phage recombinant lytic enzymes can be applied as enzybiotics in medicine as well as in biotechnology for pathogen detection or programmed cell death in bacterial expression strains. Besides, modified bacteriophages with high specificity can be applied as bioprobes in detection tools to estimate the presence of pathogens in food industry, or utilized in the control of food‐borne pathogens as part of the constructed phage‐based biosorbents.
ISSN:1364-5072
1365-2672
DOI:10.1111/jam.13207