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Actin- and Myosin-Dependent Vesicle Loading of Presynaptic Docking Sites Prior to Exocytosis

Variance analysis of postsynaptic current amplitudes suggests the presence of distinct docking sites (also called release sites) where vesicles pause before exocytosis. Docked vesicles participate in the readily releasable pool (RRP), but the relation between docking site number and RRP size remains...

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Published in:Neuron (Cambridge, Mass.) Mass.), 2016-08, Vol.91 (4), p.808-823
Main Authors: Miki, Takafumi, Malagon, Gerardo, Pulido, Camila, Llano, Isabel, Neher, Erwin, Marty, Alain
Format: Article
Language:English
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Summary:Variance analysis of postsynaptic current amplitudes suggests the presence of distinct docking sites (also called release sites) where vesicles pause before exocytosis. Docked vesicles participate in the readily releasable pool (RRP), but the relation between docking site number and RRP size remains unclear. It is also unclear whether all vesicles of the RRP are equally release competent, and what cellular mechanisms underlie RRP renewal. We address here these questions at single glutamatergic synapses, counting released vesicles using deconvolution. We find a remarkably low variance of cumulative vesicle counts during action potential trains. This, combined with Monte Carlo simulations, indicates that vesicles transit through two successive states before exocytosis, so that the RRP is up to 2-fold higher than the docking site number. The transition to the second state has a very rapid rate constant, and is specifically inhibited by latrunculin B and blebbistatin, suggesting the involvement of actin and myosin. •Variance of cumulative vesicle counts during trains is low•Release statistics suggest two-step replacement site/docking site transition•Vesicle supply from replacement site depends on actin and myosin II•Facilitation may reflect enhanced docking site loading from replacement site Miki et al. (2016) analyze statistics of vesicular release at single glutamatergic synapses during action potential trains and suggest that vesicles transit through a replacement site and a docking site before exocytosis, in a process driven by actin and myosin II.
ISSN:0896-6273
1097-4199
DOI:10.1016/j.neuron.2016.07.033