The Effect of Melatonin on Maturation, Glutathione Level and Expression of HMGB1 Gene in Brilliant Cresyl Blue (BCB) Stained Immature Oocyte

Nutrients and antioxidants in the medium of immature oocyte have a profound effect on maturation, fertilization and development of resulting embryos. In this study the effects of melatonin as an antioxidant agent on maturation, glutathione level and expression of high mobility group box-1 (HMGB1) ge...

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Bibliographic Details
Published in:Cell journal (Yakhteh) 2014, Vol.15 (4), p.294-294
Main Authors: Salimi, Maryam, Salehi, Mohammad, Farahani, Reza Masteri, Abadi, Maryam Dehghani Mohammad, Novin, Marefat Ghaffari, Nourozian, Mohsen, Hosseini, Ahmad
Format: Article
Language:English
Subjects:
Amino acids
Apoptosis
Cell culture
Cytoplasm
Deoxyribonucleic acid
DNA
Embryos
Free radicals
Genes
In vitro fertilization
Laboratory animals
Reproductive system
Science
Studies
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Summary:Nutrients and antioxidants in the medium of immature oocyte have a profound effect on maturation, fertilization and development of resulting embryos. In this study the effects of melatonin as an antioxidant agent on maturation, glutathione level and expression of high mobility group box-1 (HMGB1) gene were evaluated in immature oocytes of mice stained with brilliant cresyl blue (BCB). In this experimental study, immature oocytes were harvested from ovaries of Naval Medical Research Institute mice. Oocytes were stained with 26 μm BCB for 90 minutes and transferred to in vitro maturation medium containing varying doses of melatonin and without melatonin, for 22-24 hours. Melatonin in the concentration of 10^sup -6^ M had the most effect on maturation and HMGB1 expression of BCB+ oocytes. Meanwhile, melatonin had no effects on glutathione levels. Additionally in immature BCB- oocytes, compared to the control group, melatonin did not affect cytoplasm maturation. In vitro treatment with melatonin increases the maturation and HMGB1 expression in BCB+ immature oocytes and has no significant effect on glutathione levels.
ISSN:2228-5806
2228-5814