Loading…

Insulin-like growth factor-I gene therapy increases hippocampal neurogenesis, astrocyte branching and improves spatial memory in female aging rats

In rats, learning and memory performance decline during aging, which makes this rodent species a suitable model to evaluate therapeutic strategies of potential value for correcting age‐related cognitive deficits. Some of these strategies involve neurotrophic factors like insulin‐like growth factor‐I...

Full description

Saved in:
Bibliographic Details
Published in:The European journal of neuroscience 2016-08, Vol.44 (4), p.2120-2128
Main Authors: Pardo, Joaquín, Uriarte, Maia, Cónsole, Gloria M., Reggiani, Paula C., Outeiro, Tiago F., Morel, Gustavo R., Goya, Rodolfo G.
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c5328-39de610cd36f60c9cbbb449d0c8c38d0286ef382e18217ba9e77d4d71bf253b33
cites cdi_FETCH-LOGICAL-c5328-39de610cd36f60c9cbbb449d0c8c38d0286ef382e18217ba9e77d4d71bf253b33
container_end_page 2128
container_issue 4
container_start_page 2120
container_title The European journal of neuroscience
container_volume 44
creator Pardo, Joaquín
Uriarte, Maia
Cónsole, Gloria M.
Reggiani, Paula C.
Outeiro, Tiago F.
Morel, Gustavo R.
Goya, Rodolfo G.
description In rats, learning and memory performance decline during aging, which makes this rodent species a suitable model to evaluate therapeutic strategies of potential value for correcting age‐related cognitive deficits. Some of these strategies involve neurotrophic factors like insulin‐like growth factor‐I (IGF‐I), a powerful neuroprotective molecule in the brain. Here, we implemented 18‐day long intracerebroventricular (ICV) IGF‐I gene therapy in 28 months old Sprague–Dawley female rats, and assessed spatial memory performance in the Barnes maze. We also studied hippocampal morphology using an unbiased stereological approach. Adenovectors expressing the gene for rat IGF‐I or the reporter DsRed were used. Cerebrospinal fluid (CSF) samples were taken and IGF‐I levels determined by radioimmunoassay. At the end of the study, IGF‐I levels in the CSF were significantly higher in the experimental group than in the DsRed controls. After treatment, the IGF‐I group showed a significant improvement in spatial memory accuracy as compared with DsRed counterparts. In the dentate gyrus (DG) of the hippocampus, the IGF‐I group showed a higher number of immature neurons than the DsRed controls. The treatment increased hippocampal astrocyte branching and reduced their number in the hippocampal stratum radiatum. We conclude that the ependymal route is an effective approach to increase CSF levels of IGF‐I and that this strategy improves the accuracy of spatial memory in aging rats. The favorable effect of the treatment on DG neurogenesis and astrocyte branching in the stratum radiatum may contribute to improving memory performance in aging rats. Intracerebroventricular (ICV) Insulin‐like growth factor I (IGF‐I) gene therapy improved spatial memory accuracy in old rats, as measured in the hippocampus‐dependent Barnes maze task. Gene therapy increased immature neuron number in the hippocampal dentate gyrus. Additionaly, astrocyte number decreased , whereas their proximal branching increased in the hippocampal stratum radiatum. We conclude that ICV IGF‐I gene therapy is effective to reverse cognitive dysfunction in the aging rat.
doi_str_mv 10.1111/ejn.13278
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1815703506</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1815703506</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5328-39de610cd36f60c9cbbb449d0c8c38d0286ef382e18217ba9e77d4d71bf253b33</originalsourceid><addsrcrecordid>eNqNkc1u1DAURi0EokNhwQsgL0EirX_inyxRVcqMqgJSge4sx7mZcZvYqZ1Q5jV4YjJM2x0S3tzNOUeWPoReU3JE53cM1-GIcqb0E7SgpSRFJaR-ihakErzQVF4doBc5XxNCtCzFc3TAFNW6pGKBfi9Dnjofis7fAF6neDducGvdGFOxxGsIgMcNJDtssQ8ugc2Q8cYPQ3S2H2yHA0wp7rjs83ts85ii246A62SD2_iwxjY02PdDij9nNQ929LPWQx_Trolb6G0H2K53bLJjfometbbL8Or-HqJvH08vTz4V55_PlicfzgsnONMFrxqQlLiGy1YSV7m6rsuyaojTjuuGMC2h5ZoB1Yyq2lagVFM2itYtE7zm_BC93Xfnr91OkEfT--yg62yAOGVDNRWKcEHk_6CsLBknYkbf7VGXYs4JWjMk39u0NZSY3VpmXsv8XWtm39xnp7qH5pF8mGcGjvfAne9g---SOV1dPCSLveHzCL8eDZtujFRcCfPj4sxItvr65fvqylzyP_RjsMg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1812442305</pqid></control><display><type>article</type><title>Insulin-like growth factor-I gene therapy increases hippocampal neurogenesis, astrocyte branching and improves spatial memory in female aging rats</title><source>Wiley-Blackwell Read &amp; Publish Collection</source><creator>Pardo, Joaquín ; Uriarte, Maia ; Cónsole, Gloria M. ; Reggiani, Paula C. ; Outeiro, Tiago F. ; Morel, Gustavo R. ; Goya, Rodolfo G.</creator><contributor>Klausberger, Thomas ; Klausberger, Thomas</contributor><creatorcontrib>Pardo, Joaquín ; Uriarte, Maia ; Cónsole, Gloria M. ; Reggiani, Paula C. ; Outeiro, Tiago F. ; Morel, Gustavo R. ; Goya, Rodolfo G. ; Klausberger, Thomas ; Klausberger, Thomas</creatorcontrib><description>In rats, learning and memory performance decline during aging, which makes this rodent species a suitable model to evaluate therapeutic strategies of potential value for correcting age‐related cognitive deficits. Some of these strategies involve neurotrophic factors like insulin‐like growth factor‐I (IGF‐I), a powerful neuroprotective molecule in the brain. Here, we implemented 18‐day long intracerebroventricular (ICV) IGF‐I gene therapy in 28 months old Sprague–Dawley female rats, and assessed spatial memory performance in the Barnes maze. We also studied hippocampal morphology using an unbiased stereological approach. Adenovectors expressing the gene for rat IGF‐I or the reporter DsRed were used. Cerebrospinal fluid (CSF) samples were taken and IGF‐I levels determined by radioimmunoassay. At the end of the study, IGF‐I levels in the CSF were significantly higher in the experimental group than in the DsRed controls. After treatment, the IGF‐I group showed a significant improvement in spatial memory accuracy as compared with DsRed counterparts. In the dentate gyrus (DG) of the hippocampus, the IGF‐I group showed a higher number of immature neurons than the DsRed controls. The treatment increased hippocampal astrocyte branching and reduced their number in the hippocampal stratum radiatum. We conclude that the ependymal route is an effective approach to increase CSF levels of IGF‐I and that this strategy improves the accuracy of spatial memory in aging rats. The favorable effect of the treatment on DG neurogenesis and astrocyte branching in the stratum radiatum may contribute to improving memory performance in aging rats. Intracerebroventricular (ICV) Insulin‐like growth factor I (IGF‐I) gene therapy improved spatial memory accuracy in old rats, as measured in the hippocampus‐dependent Barnes maze task. Gene therapy increased immature neuron number in the hippocampal dentate gyrus. Additionaly, astrocyte number decreased , whereas their proximal branching increased in the hippocampal stratum radiatum. We conclude that ICV IGF‐I gene therapy is effective to reverse cognitive dysfunction in the aging rat.</description><identifier>ISSN: 0953-816X</identifier><identifier>EISSN: 1460-9568</identifier><identifier>DOI: 10.1111/ejn.13278</identifier><identifier>PMID: 27188415</identifier><language>eng</language><publisher>France: Blackwell Publishing Ltd</publisher><subject>aging ; Animals ; Astrocytes - metabolism ; Barnes maze ; Cognition - physiology ; Cognition Disorders - genetics ; Cognition Disorders - metabolism ; Cognition Disorders - therapy ; Female ; gene therapy ; Genetic Therapy ; hippocampal morphology ; Hippocampus - cytology ; Hippocampus - metabolism ; IGF-I ; Insulin-Like Growth Factor I - genetics ; Insulin-Like Growth Factor I - metabolism ; Memory Disorders - genetics ; Memory Disorders - therapy ; Neurogenesis - physiology ; Rats, Sprague-Dawley ; spatial memory ; Spatial Memory - physiology</subject><ispartof>The European journal of neuroscience, 2016-08, Vol.44 (4), p.2120-2128</ispartof><rights>2016 Federation of European Neuroscience Societies and John Wiley &amp; Sons Ltd</rights><rights>2016 Federation of European Neuroscience Societies and John Wiley &amp; Sons Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5328-39de610cd36f60c9cbbb449d0c8c38d0286ef382e18217ba9e77d4d71bf253b33</citedby><cites>FETCH-LOGICAL-c5328-39de610cd36f60c9cbbb449d0c8c38d0286ef382e18217ba9e77d4d71bf253b33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27188415$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Klausberger, Thomas</contributor><contributor>Klausberger, Thomas</contributor><creatorcontrib>Pardo, Joaquín</creatorcontrib><creatorcontrib>Uriarte, Maia</creatorcontrib><creatorcontrib>Cónsole, Gloria M.</creatorcontrib><creatorcontrib>Reggiani, Paula C.</creatorcontrib><creatorcontrib>Outeiro, Tiago F.</creatorcontrib><creatorcontrib>Morel, Gustavo R.</creatorcontrib><creatorcontrib>Goya, Rodolfo G.</creatorcontrib><title>Insulin-like growth factor-I gene therapy increases hippocampal neurogenesis, astrocyte branching and improves spatial memory in female aging rats</title><title>The European journal of neuroscience</title><addtitle>Eur J Neurosci</addtitle><description>In rats, learning and memory performance decline during aging, which makes this rodent species a suitable model to evaluate therapeutic strategies of potential value for correcting age‐related cognitive deficits. Some of these strategies involve neurotrophic factors like insulin‐like growth factor‐I (IGF‐I), a powerful neuroprotective molecule in the brain. Here, we implemented 18‐day long intracerebroventricular (ICV) IGF‐I gene therapy in 28 months old Sprague–Dawley female rats, and assessed spatial memory performance in the Barnes maze. We also studied hippocampal morphology using an unbiased stereological approach. Adenovectors expressing the gene for rat IGF‐I or the reporter DsRed were used. Cerebrospinal fluid (CSF) samples were taken and IGF‐I levels determined by radioimmunoassay. At the end of the study, IGF‐I levels in the CSF were significantly higher in the experimental group than in the DsRed controls. After treatment, the IGF‐I group showed a significant improvement in spatial memory accuracy as compared with DsRed counterparts. In the dentate gyrus (DG) of the hippocampus, the IGF‐I group showed a higher number of immature neurons than the DsRed controls. The treatment increased hippocampal astrocyte branching and reduced their number in the hippocampal stratum radiatum. We conclude that the ependymal route is an effective approach to increase CSF levels of IGF‐I and that this strategy improves the accuracy of spatial memory in aging rats. The favorable effect of the treatment on DG neurogenesis and astrocyte branching in the stratum radiatum may contribute to improving memory performance in aging rats. Intracerebroventricular (ICV) Insulin‐like growth factor I (IGF‐I) gene therapy improved spatial memory accuracy in old rats, as measured in the hippocampus‐dependent Barnes maze task. Gene therapy increased immature neuron number in the hippocampal dentate gyrus. Additionaly, astrocyte number decreased , whereas their proximal branching increased in the hippocampal stratum radiatum. We conclude that ICV IGF‐I gene therapy is effective to reverse cognitive dysfunction in the aging rat.</description><subject>aging</subject><subject>Animals</subject><subject>Astrocytes - metabolism</subject><subject>Barnes maze</subject><subject>Cognition - physiology</subject><subject>Cognition Disorders - genetics</subject><subject>Cognition Disorders - metabolism</subject><subject>Cognition Disorders - therapy</subject><subject>Female</subject><subject>gene therapy</subject><subject>Genetic Therapy</subject><subject>hippocampal morphology</subject><subject>Hippocampus - cytology</subject><subject>Hippocampus - metabolism</subject><subject>IGF-I</subject><subject>Insulin-Like Growth Factor I - genetics</subject><subject>Insulin-Like Growth Factor I - metabolism</subject><subject>Memory Disorders - genetics</subject><subject>Memory Disorders - therapy</subject><subject>Neurogenesis - physiology</subject><subject>Rats, Sprague-Dawley</subject><subject>spatial memory</subject><subject>Spatial Memory - physiology</subject><issn>0953-816X</issn><issn>1460-9568</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNqNkc1u1DAURi0EokNhwQsgL0EirX_inyxRVcqMqgJSge4sx7mZcZvYqZ1Q5jV4YjJM2x0S3tzNOUeWPoReU3JE53cM1-GIcqb0E7SgpSRFJaR-ihakErzQVF4doBc5XxNCtCzFc3TAFNW6pGKBfi9Dnjofis7fAF6neDducGvdGFOxxGsIgMcNJDtssQ8ugc2Q8cYPQ3S2H2yHA0wp7rjs83ts85ii246A62SD2_iwxjY02PdDij9nNQ929LPWQx_Trolb6G0H2K53bLJjfometbbL8Or-HqJvH08vTz4V55_PlicfzgsnONMFrxqQlLiGy1YSV7m6rsuyaojTjuuGMC2h5ZoB1Yyq2lagVFM2itYtE7zm_BC93Xfnr91OkEfT--yg62yAOGVDNRWKcEHk_6CsLBknYkbf7VGXYs4JWjMk39u0NZSY3VpmXsv8XWtm39xnp7qH5pF8mGcGjvfAne9g---SOV1dPCSLveHzCL8eDZtujFRcCfPj4sxItvr65fvqylzyP_RjsMg</recordid><startdate>201608</startdate><enddate>201608</enddate><creator>Pardo, Joaquín</creator><creator>Uriarte, Maia</creator><creator>Cónsole, Gloria M.</creator><creator>Reggiani, Paula C.</creator><creator>Outeiro, Tiago F.</creator><creator>Morel, Gustavo R.</creator><creator>Goya, Rodolfo G.</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TK</scope></search><sort><creationdate>201608</creationdate><title>Insulin-like growth factor-I gene therapy increases hippocampal neurogenesis, astrocyte branching and improves spatial memory in female aging rats</title><author>Pardo, Joaquín ; Uriarte, Maia ; Cónsole, Gloria M. ; Reggiani, Paula C. ; Outeiro, Tiago F. ; Morel, Gustavo R. ; Goya, Rodolfo G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5328-39de610cd36f60c9cbbb449d0c8c38d0286ef382e18217ba9e77d4d71bf253b33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>aging</topic><topic>Animals</topic><topic>Astrocytes - metabolism</topic><topic>Barnes maze</topic><topic>Cognition - physiology</topic><topic>Cognition Disorders - genetics</topic><topic>Cognition Disorders - metabolism</topic><topic>Cognition Disorders - therapy</topic><topic>Female</topic><topic>gene therapy</topic><topic>Genetic Therapy</topic><topic>hippocampal morphology</topic><topic>Hippocampus - cytology</topic><topic>Hippocampus - metabolism</topic><topic>IGF-I</topic><topic>Insulin-Like Growth Factor I - genetics</topic><topic>Insulin-Like Growth Factor I - metabolism</topic><topic>Memory Disorders - genetics</topic><topic>Memory Disorders - therapy</topic><topic>Neurogenesis - physiology</topic><topic>Rats, Sprague-Dawley</topic><topic>spatial memory</topic><topic>Spatial Memory - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pardo, Joaquín</creatorcontrib><creatorcontrib>Uriarte, Maia</creatorcontrib><creatorcontrib>Cónsole, Gloria M.</creatorcontrib><creatorcontrib>Reggiani, Paula C.</creatorcontrib><creatorcontrib>Outeiro, Tiago F.</creatorcontrib><creatorcontrib>Morel, Gustavo R.</creatorcontrib><creatorcontrib>Goya, Rodolfo G.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Neurosciences Abstracts</collection><jtitle>The European journal of neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pardo, Joaquín</au><au>Uriarte, Maia</au><au>Cónsole, Gloria M.</au><au>Reggiani, Paula C.</au><au>Outeiro, Tiago F.</au><au>Morel, Gustavo R.</au><au>Goya, Rodolfo G.</au><au>Klausberger, Thomas</au><au>Klausberger, Thomas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Insulin-like growth factor-I gene therapy increases hippocampal neurogenesis, astrocyte branching and improves spatial memory in female aging rats</atitle><jtitle>The European journal of neuroscience</jtitle><addtitle>Eur J Neurosci</addtitle><date>2016-08</date><risdate>2016</risdate><volume>44</volume><issue>4</issue><spage>2120</spage><epage>2128</epage><pages>2120-2128</pages><issn>0953-816X</issn><eissn>1460-9568</eissn><abstract>In rats, learning and memory performance decline during aging, which makes this rodent species a suitable model to evaluate therapeutic strategies of potential value for correcting age‐related cognitive deficits. Some of these strategies involve neurotrophic factors like insulin‐like growth factor‐I (IGF‐I), a powerful neuroprotective molecule in the brain. Here, we implemented 18‐day long intracerebroventricular (ICV) IGF‐I gene therapy in 28 months old Sprague–Dawley female rats, and assessed spatial memory performance in the Barnes maze. We also studied hippocampal morphology using an unbiased stereological approach. Adenovectors expressing the gene for rat IGF‐I or the reporter DsRed were used. Cerebrospinal fluid (CSF) samples were taken and IGF‐I levels determined by radioimmunoassay. At the end of the study, IGF‐I levels in the CSF were significantly higher in the experimental group than in the DsRed controls. After treatment, the IGF‐I group showed a significant improvement in spatial memory accuracy as compared with DsRed counterparts. In the dentate gyrus (DG) of the hippocampus, the IGF‐I group showed a higher number of immature neurons than the DsRed controls. The treatment increased hippocampal astrocyte branching and reduced their number in the hippocampal stratum radiatum. We conclude that the ependymal route is an effective approach to increase CSF levels of IGF‐I and that this strategy improves the accuracy of spatial memory in aging rats. The favorable effect of the treatment on DG neurogenesis and astrocyte branching in the stratum radiatum may contribute to improving memory performance in aging rats. Intracerebroventricular (ICV) Insulin‐like growth factor I (IGF‐I) gene therapy improved spatial memory accuracy in old rats, as measured in the hippocampus‐dependent Barnes maze task. Gene therapy increased immature neuron number in the hippocampal dentate gyrus. Additionaly, astrocyte number decreased , whereas their proximal branching increased in the hippocampal stratum radiatum. We conclude that ICV IGF‐I gene therapy is effective to reverse cognitive dysfunction in the aging rat.</abstract><cop>France</cop><pub>Blackwell Publishing Ltd</pub><pmid>27188415</pmid><doi>10.1111/ejn.13278</doi><tpages>9</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0953-816X
ispartof The European journal of neuroscience, 2016-08, Vol.44 (4), p.2120-2128
issn 0953-816X
1460-9568
language eng
recordid cdi_proquest_miscellaneous_1815703506
source Wiley-Blackwell Read & Publish Collection
subjects aging
Animals
Astrocytes - metabolism
Barnes maze
Cognition - physiology
Cognition Disorders - genetics
Cognition Disorders - metabolism
Cognition Disorders - therapy
Female
gene therapy
Genetic Therapy
hippocampal morphology
Hippocampus - cytology
Hippocampus - metabolism
IGF-I
Insulin-Like Growth Factor I - genetics
Insulin-Like Growth Factor I - metabolism
Memory Disorders - genetics
Memory Disorders - therapy
Neurogenesis - physiology
Rats, Sprague-Dawley
spatial memory
Spatial Memory - physiology
title Insulin-like growth factor-I gene therapy increases hippocampal neurogenesis, astrocyte branching and improves spatial memory in female aging rats
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T02%3A56%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Insulin-like%20growth%20factor-I%20gene%20therapy%20increases%20hippocampal%20neurogenesis,%20astrocyte%20branching%20and%20improves%20spatial%20memory%20in%20female%20aging%20rats&rft.jtitle=The%20European%20journal%20of%20neuroscience&rft.au=Pardo,%20Joaqu%C3%ADn&rft.date=2016-08&rft.volume=44&rft.issue=4&rft.spage=2120&rft.epage=2128&rft.pages=2120-2128&rft.issn=0953-816X&rft.eissn=1460-9568&rft_id=info:doi/10.1111/ejn.13278&rft_dat=%3Cproquest_cross%3E1815703506%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c5328-39de610cd36f60c9cbbb449d0c8c38d0286ef382e18217ba9e77d4d71bf253b33%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1812442305&rft_id=info:pmid/27188415&rfr_iscdi=true