Production and identification of iturin A lipopeptide from Bacillus methyltrophicus TEB1 for control of Phoma tracheiphila

A lipopeptide‐producing endophytic Bacillus methyltrophicus TEB1 strain exhibited potent antifungal activity against Phoma tracheiphila. Lipopeptide production started at the early growth phase plateaued after 36 h of culture where it reduced the mycelium growth by 80%. The crude lipopeptide extract...

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Bibliographic Details
Published in:Journal of basic microbiology 2016-08, Vol.56 (8), p.864-871
Main Authors: Kalai-Grami, Leila, Karkouch, Ines, Naili, Omar, Slimene, Imen Ben, Elkahoui, Salem, Zekri, Roudaina Ben, Touati, Ines, Mnari-Hattab, Monia, Hajlaoui, Mohamed Rabeh, Limam, Ferid
Format: Article
Language:English
Subjects:
Antifungal Agents - pharmacology
Ascomycota - drug effects
Ascomycota - growth & development
Bacillus
Bacillus - genetics
Bacillus - metabolism
Bacillus methyltrophicus
Biological Control Agents - pharmacology
Iturin A
Lipopeptides
Lipopeptides - biosynthesis
Lipopeptides - pharmacology
Mal secco
Mass Spectrometry
Microbial Sensitivity Tests
Mycelium - drug effects
Mycelium - growth & development
Peptides, Cyclic - biosynthesis
Peptides, Cyclic - pharmacology
Phoma
Phoma tracheiphila
Plant Diseases - microbiology
Spores, Fungal - drug effects
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Summary:A lipopeptide‐producing endophytic Bacillus methyltrophicus TEB1 strain exhibited potent antifungal activity against Phoma tracheiphila. Lipopeptide production started at the early growth phase plateaued after 36 h of culture where it reduced the mycelium growth by 80%. The crude lipopeptide extract harvested at the stationary phase efficiently inhibited the growth of P. tracheiphila mycelium and MIC values displaying 50 and 90% inhibition of conidia germination were around 47.5 and 100 μg ml−1, respectively. Increasing lipopeptide extract till 3 mg ml−1 induced 10% swelling and 3% crumbling of P. tracheiphila conidia whereas 5 mg ml−1 induced 40% swelling and 20% crumbling. Mass spectrometry analysis of the lipopeptide extract indicated that surfactin production took place from 12 to 20 h, iturin A from 16 to 72 h, and fengycin from 12 to 72 h and that the main active compound against P. tracheiphila was identified as C15 iturin A lipopeptide. Iturin A appeared as a potential biological control agent able to substitute the currently used chemical pesticides in agriculture.
ISSN:0233-111X
1521-4028
DOI:10.1002/jobm.201500683