The rs6983267 SNP and long non-coding RNA CARLo-5 are associated with endometrial carcinoma

• Published in: Environmental and molecular mutagenesis 2016-08, Vol.57 (7), p.508-515
• Main Authors: Zhao, Xiwa, Wei, Xurui, Zhao, Lianmei, Shi, Li, Cheng, Jianxin, Kang, Shan, Zhang, Hui, Zhang, Jun, Li, Li, Zhang, Haibo, Zhao, Wei
• Format: Article
• Language: English
• Subjects: Adult; Aged; CARLo-5; Case-Control Studies; Chromosomes, Human, Pair 8 - genetics; DNA - genetics; endometrial carcinoma; Endometrial Neoplasms - genetics; Female; Gene Expression Regulation, Neoplastic; Gene Frequency; Genetic Predisposition to Disease; Genotyping Techniques; Humans; Kaplan-Meier Estimate; Middle Aged; Polymorphism, Single Nucleotide; Prognosis; Real-Time Polymerase Chain Reaction; RNA, Long Noncoding - genetics; rs6983267; susceptibility; Up-Regulation
• ISSN: 0893-6692; 1098-2280
• DOI: 10.1002/em.22031

The single nucleotide polymorphism (SNP) rs6983267 and cancer‐associated region long non‐coding RNA (CARLo‐5) are associated with various human cancers. This study aimed to investigate the expression of CARLo‐5 in endometrial carcinoma (EC) and its relationship with clinicopathological features and patient survival. The association of the rs6983267 SNP with EC risk and its involvement in the regulation of CARLo‐5 expression in EC were investigated. The rs6983267 SNP was genotyped by polymerase chain reaction (PCR) and ligase detection reaction in 543 EC patients and 584 controls. The expression of CARLo‐5 in 108 EC tissues and 66 normal endometrial tissues (NETs) was determined using quantitative real‐time PCR. The genotype and allele distributions of the rs6983267 SNP differed significantly between patients and controls. There was a significant correlation between the rs6983267 genotypes and lymph node metastasis of EC patients (P = 0.026). CARLo‐5 expression was significantly higher in EC tissues than in NETs (P