Metabolite fingerprinting, pathway analyses, and bioactivity correlations for plant species belonging to the Cornaceae, Fabaceae, and Rosaceae families

• Published in: Plant cell reports 2016-09, Vol.35 (9), p.1917-1931
• Main Authors: Son, Su Young, Kim, Na Kyung, Lee, Sunmin, Singh, Digar, Kim, Ga Ryun, Lee, Jong Seok, Yang, Hee-sun, Yeo, Joohong, Lee, Sarah, Lee, Choong Hwan
• Format: Article
• Language: English
• Subjects: Amino acids; Antioxidants; Antioxidants - metabolism; Biomedical and Life Sciences; Biotechnology; Cell Biology; Cornaceae; Cornaceae - metabolism; Discriminant Analysis; Fabaceae; Fabaceae - metabolism; Gas chromatography; Least-Squares Analysis; Life Sciences; Liquid chromatography; Mass spectrometry; Metabolic Networks and Pathways; Metabolites; Metabolome; Metabolomics - methods; Original Article; Phenols; Plant Biochemistry; Plant extracts; Plant Sciences; Plant species; Rosaceae; Rosaceae - metabolism; Secondary Metabolism; Species Specificity
• ISSN: 0721-7714; 1432-203X
• DOI: 10.1007/s00299-016-2006-y

Key message A multi-parallel approach gauging the mass spectrometry-based metabolite fingerprinting coupled with bioactivity and pathway evaluations could serve as an efficacious tool for inferring plant taxonomic orders. Thirty-four species from three plant families, namely Cornaceae (7), Fabaceae (9), and Rosaceae (18) were subjected to metabolite profiling using gas chromatography–time-of-flight-mass spectrometry (GC–TOF-MS) and ultrahigh performance liquid chromatography–linear trap quadrupole-ion trap-mass spectrometry (UHPLC–LTQ-IT-MS/MS), followed by multivariate analyses to determine the metabolites characteristic of these families. The partial least squares discriminant analysis (PLS-DA) revealed the distinct clustering pattern of metabolites for each family. The pathway analysis further highlighted the relatively higher proportions of flavonols and ellagitannins in the Cornaceae family than in the other two families. Higher levels of phenolic acids and flavan-3-ols were observed among species from the Rosaceae family, while amino acids, flavones, and isoflavones were more abundant among the Fabaceae family members. The antioxidant activities of plant extracts were measured using ABTS, DPPH, and FRAP assays, and indicated that extracts from the Rosaceae family had the highest activity, followed by those from Cornaceae and Fabaceae. The correlation map analysis positively links the proportional concentration of metabolites with their relative antioxidant activities, particularly in Cornaceae and Rosaceae. This work highlights the pre-eminence of the multi-parallel approach involving metabolite profiling and bioactivity evaluations coupled with metabolic pathways as an efficient methodology for the evaluation of plant phylogenies.