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Molecular characterization of Staphylococcus aureus isolates causing skin and soft tissue infections in patients from Malakand, Pakistan

Comparatively few studies have been published describing Staphylococcus aureus /MRSA epidemiology in Central Asia including Pakistan. Here, we report the genotyping of Staphylococcus aureus strains (that include both methicillin-susceptible and methicillin-resistant Staphylococcus aureus ) from comm...

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Bibliographic Details
Published in:European journal of clinical microbiology & infectious diseases 2016-09, Vol.35 (9), p.1541-1547
Main Authors: Madzgalla, S., Syed, M. A., Khan, M. A., Rehman, S. S., Müller, E., Reissig, A., Ehricht, R., Monecke, S.
Format: Article
Language:English
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Summary:Comparatively few studies have been published describing Staphylococcus aureus /MRSA epidemiology in Central Asia including Pakistan. Here, we report the genotyping of Staphylococcus aureus strains (that include both methicillin-susceptible and methicillin-resistant Staphylococcus aureus ) from community- and hospital-acquired skin and soft-tissue infections in a tertiary care hospital in the Malakand district of the Khyber Pakhtunkhwa Province of Pakistan. Forty-five isolates of Staphylococcus aureus were characterized by microarray hybridization. Twenty isolates (44 %) were MRSA, whereas 22 (49 %) were PVL-positive. Fourteen isolates (31 %) harboured both mecA and PVL genes. The dominant clones were CC121-MSSA ( n  = 15, 33 %) and the PVL-positive “Bengal Bay Clone” (ST772-MRSA-V; n  = 13, 29 %). The PVL-positive CC8-MRSA-IV strain “USA300” was found once. The pandemic ST239-MRSA-III strain was absent, although it has previously been observed in Pakistan. These observations require a re-assessment of schemes for initial antibiotic therapy to cover MRSA and they emphasise the need for a rapid and non-molecular test for PVL.
ISSN:0934-9723
1435-4373
DOI:10.1007/s10096-016-2695-8