In vivo neutralization of endogenous brain fractalkine increases hippocampal TNFα and 8-isoprostane production induced by intracerebroventricular injection of LPS

Fractalkine is a chemokine widely and constitutively expressed in the brain and, as suggested by in vitro studies, it is involved in brain inflammatory responses. In this study, we have investigated the in vivo anti-inflammatory potential of fractalkine in a model of neuroinflammation induced by int...

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Bibliographic Details
Published in:Journal of neuroimmunology 2001-04, Vol.115 (1), p.135-143
Main Authors: Zujovic, Violetta, Schussler, Nathalie, Jourdain, Danièle, Duverger, Danielle, Taupin, Véronique
Format: Article
Language:English
Subjects:
8-Isoprostane
8-Isoprostanes
Fractalkine
Intracerebroventricular injection
LPS
TNFα
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Summary:Fractalkine is a chemokine widely and constitutively expressed in the brain and, as suggested by in vitro studies, it is involved in brain inflammatory responses. In this study, we have investigated the in vivo anti-inflammatory potential of fractalkine in a model of neuroinflammation induced by intracerebroventricular injection of lipopolysaccharide (LPS) in rats. LPS induces a rapid and acute production of the pro-inflammatory cytokine, TNFα, in hippocampus and cerebrospinal fluid (CSF), and an increase of 8-isoprostane levels, a marker of oxidative stress, in hippocampus. Although intracerebroventricular injection of fractalkine has no effect on TNFα and 8-isoprostane production, neutralization of endogenous fractalkine within the brain with a specific anti-fractalkine antibody potentiates LPS effects. These data emphasize the involvement of constitutive brain fractalkine in the control of inflammatory reaction in CNS.
ISSN:0165-5728
1872-8421
DOI:10.1016/S0165-5728(01)00259-4