FRET-Based Mito-Specific Fluorescent Probe for Ratiometric Detection and Imaging of Endogenous Peroxynitrite: Dyad of Cy3 and Cy5

Peroxynitrite (OONO–) is profoundly implicated in health and disease. The physiological and pathological outcome of OONO– is related to its local concentration, and hence, a reliable OONO– assay is highly desired. We have developed a FRET-based small-molecule fluorescent probe (PNCy3Cy5), harnessing...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2016-08, Vol.138 (34), p.10778-10781
Main Authors: Jia, Xiaotong, Chen, Qiangqiang, Yang, Yingfang, Tang, Yao, Wang, Rui, Xu, Yufang, Zhu, Weiping, Qian, Xuhong
Format: Article
Language:English
Subjects:
Animals
Carbocyanines - chemistry
Fluorescence Resonance Energy Transfer
Fluorescent Dyes - chemistry
Mice
Mitochondria - metabolism
Optical Imaging
Peroxynitrous Acid - chemistry
Peroxynitrous Acid - metabolism
RAW 264.7 Cells
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Summary:Peroxynitrite (OONO–) is profoundly implicated in health and disease. The physiological and pathological outcome of OONO– is related to its local concentration, and hence, a reliable OONO– assay is highly desired. We have developed a FRET-based small-molecule fluorescent probe (PNCy3Cy5), harnessing the differential reactivity of Cy3 and Cy5 toward OONO– by fine-tuning. It exhibits high detection sensitivity and yields a ratiometric fluorescent signal. We have exemplified that it can be applied in semiquantitative determination of OONO– in living cells. Notably, it specifically localizes in mitochondria, where endogenous OONO– is predominantly generated. Thus, PNCy3Cy5 is a promising molecular tool for peroxynitrite biology.
ISSN:0002-7863
1520-5126
DOI:10.1021/jacs.6b06398