Salt Stress Reveals a New Role for ARGONAUTE1 in miRNA Biogenesis at the Transcriptional and Posttranscriptional Levels

Plants as sessile organisms have developed prompt response mechanisms to react to rapid environmental changes. In addition to the transcriptional regulation of gene expression, microRNAs (miRNAs) are key posttranscriptional regulators of the plant stress response. We show here that the expression le...

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Published in:Plant physiology (Bethesda) 2016-09, Vol.172 (1), p.297-312
Main Authors: Dolata, Jakub, Bajczyk, Mateusz, Bielewicz, Dawid, Niedojadlo, Katarzyna, Niedojadlo, Janusz, Pietrykowska, Halina, Walczak, Weronika, Szweykowska-Kulinska, Zofia, Jarmolowski, Artur
Format: Article
Language:English
Subjects:
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Argonaute Proteins - genetics
Argonaute Proteins - metabolism
Blotting, Western
Cell Nucleus - drug effects
Cell Nucleus - genetics
Down-Regulation - drug effects
Gene Expression Regulation, Plant - drug effects
Gene Expression Regulation, Plant - genetics
GENES, DEVELOPMENT, AND EVOLUTION
MicroRNAs - genetics
Plants, Genetically Modified
Reverse Transcriptase Polymerase Chain Reaction
RNA Stability - drug effects
RNA Stability - genetics
RNA, Plant - genetics
Salinity
Sodium Chloride - pharmacology
Stress, Physiological
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Summary:Plants as sessile organisms have developed prompt response mechanisms to react to rapid environmental changes. In addition to the transcriptional regulation of gene expression, microRNAs (miRNAs) are key posttranscriptional regulators of the plant stress response. We show here that the expression levels of many miRNAs were regulated under salt stress conditions. This regulation occurred at the transcriptional and posttranscriptional levels. During salinity stress, the levels of miRNA161 and miRNA173 increased, while the expression of pri-miRNA161 and pri-miRNA173 was down-regulated. Under salt stress conditions, miRNA161 and miRNA173 were stabilized in the cytoplasm, and the expressions of MIR161 and MIR173 were negatively regulated in the nucleus. ARGONAUTE1 (AGO1) participated in both processes. We demonstrated that AGO1 cotranscriptionally controlled the expression of MIR161 and MIR173 in the nucleus. Our results suggests that AGO1 interacts with chromatin at MIR161 and MIR173 loci and causes the disassembly of the transcriptional complex, releasing short and unpolyadenylated transcripts.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.16.00830