Improvement of Electrochemical Response of Cocaine Sensors Based on DNA Aptamer by Heat Treatment

We report on a biosensor for cocaine based on the conformation change of DNA aptamer by capturing the cocaine molecules. The oxidation current of ferrocene conjugated on the terminal end of aptamer immobilized on an Au electrode increased with increasing cocaine concentration. The sensor response ha...

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Bibliographic Details
Published in:Analytical Sciences 2016/04/10, Vol.32(4), pp.469-472
Main Authors: ARIMOTO, Satoshi, SHIMONO, Ken, YASUKAWA, Tomoyuki, MIZUTANI, Fumio, YOSHIOKA, Toshihiko
Format: Article
Language:English
Subjects:
Analytical Chemistry
Aptamers, Nucleotide - chemistry
Biosensing Techniques
Chemistry
Cocaine - analysis
cocaine detection
Deoxyribonucleic acid
electrochemical measurements
Electrochemical Techniques
Electrodes
ferrocene conjugated DNA aptamer
Heat treatment
Hot Temperature
Immobilization
Mathematical analysis
Narcotics
Sensors
Terminals
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Summary:We report on a biosensor for cocaine based on the conformation change of DNA aptamer by capturing the cocaine molecules. The oxidation current of ferrocene conjugated on the terminal end of aptamer immobilized on an Au electrode increased with increasing cocaine concentration. The sensor response has been improved by a simple heat treatment after immobilization, since the aggregates of DNA aptamer generated during the immobilization step could be dissociated and rearranged on the electrode.
ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.32.469