Improvement of Electrochemical Response of Cocaine Sensors Based on DNA Aptamer by Heat Treatment

We report on a biosensor for cocaine based on the conformation change of DNA aptamer by capturing the cocaine molecules. The oxidation current of ferrocene conjugated on the terminal end of aptamer immobilized on an Au electrode increased with increasing cocaine concentration. The sensor response ha...

Full description

Saved in:
Bibliographic Details
Published in:Analytical Sciences 2016/04/10, Vol.32(4), pp.469-472
Main Authors: ARIMOTO, Satoshi, SHIMONO, Ken, YASUKAWA, Tomoyuki, MIZUTANI, Fumio, YOSHIOKA, Toshihiko
Format: Article
Language:English
Subjects:
Analytical Chemistry
Aptamers, Nucleotide - chemistry
Biosensing Techniques
Chemistry
Cocaine - analysis
cocaine detection
Deoxyribonucleic acid
electrochemical measurements
Electrochemical Techniques
Electrodes
ferrocene conjugated DNA aptamer
Heat treatment
Hot Temperature
Immobilization
Mathematical analysis
Narcotics
Sensors
Terminals
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c745t-88413066da4f5fe20ea71d0c7f52521d7f7bdf51bdb3607951dcf37d73f9b9753
cites cdi_FETCH-LOGICAL-c745t-88413066da4f5fe20ea71d0c7f52521d7f7bdf51bdb3607951dcf37d73f9b9753
container_end_page 472
container_issue 4
container_start_page 469
container_title Analytical Sciences
container_volume 32
creator ARIMOTO, Satoshi
SHIMONO, Ken
YASUKAWA, Tomoyuki
MIZUTANI, Fumio
YOSHIOKA, Toshihiko
description We report on a biosensor for cocaine based on the conformation change of DNA aptamer by capturing the cocaine molecules. The oxidation current of ferrocene conjugated on the terminal end of aptamer immobilized on an Au electrode increased with increasing cocaine concentration. The sensor response has been improved by a simple heat treatment after immobilization, since the aggregates of DNA aptamer generated during the immobilization step could be dissociated and rearranged on the electrode.
doi_str_mv 10.2116/analsci.32.469
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1815983898</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1815983898</sourcerecordid><originalsourceid>FETCH-LOGICAL-c745t-88413066da4f5fe20ea71d0c7f52521d7f7bdf51bdb3607951dcf37d73f9b9753</originalsourceid><addsrcrecordid>eNqNkc9rFDEcxYModm29epSAFy-zze9MjutabaG0oO05ZDLftLPMTNZkVuh_b4ZdFxVED0kO7_Pe9xseQm8oWTJK1bkbXZ99t-RsKZR5hhaUi7piTKjnaEEMJZXigpygVzlvCKGsZuwlOmGaKK4ZWyB3NWxT_A4DjBOOAV_04KcU_SMMnXc9_gJ5G8cMs7aO3nUj4K8w5pgy_uAytDiO-OPNCq-2kxsg4eYJX4Kb8F0q95x6hl6EsiO8Pryn6P7Txd36srq-_Xy1Xl1XXgs5VXUtKCdKtU4EGYARcJq2xOsgmWS01UE3bZC0aRuuiDaStj5w3WoeTGO05Kfo_T63_OfbDvJkhy576Hs3QtxlS2sqTc3rcv6JaiOEqZU2_4HWRFJqKC_ouz_QTdyluZ-Z0oRpqebZyz3lU8w5QbDb1A0uPVlK7FypPVRqObOl0mJ4e4jdNQO0R_xnhwU43wO5SOMDpF_m_i1ytXds8uQe4Bjp0tT5Hn7DD56j5h9dsjDyHztQxBU</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1787027568</pqid></control><display><type>article</type><title>Improvement of Electrochemical Response of Cocaine Sensors Based on DNA Aptamer by Heat Treatment</title><source>J-STAGE Free</source><source>Springer Nature</source><creator>ARIMOTO, Satoshi ; SHIMONO, Ken ; YASUKAWA, Tomoyuki ; MIZUTANI, Fumio ; YOSHIOKA, Toshihiko</creator><creatorcontrib>ARIMOTO, Satoshi ; SHIMONO, Ken ; YASUKAWA, Tomoyuki ; MIZUTANI, Fumio ; YOSHIOKA, Toshihiko</creatorcontrib><description>We report on a biosensor for cocaine based on the conformation change of DNA aptamer by capturing the cocaine molecules. The oxidation current of ferrocene conjugated on the terminal end of aptamer immobilized on an Au electrode increased with increasing cocaine concentration. The sensor response has been improved by a simple heat treatment after immobilization, since the aggregates of DNA aptamer generated during the immobilization step could be dissociated and rearranged on the electrode.</description><identifier>ISSN: 0910-6340</identifier><identifier>EISSN: 1348-2246</identifier><identifier>DOI: 10.2116/analsci.32.469</identifier><identifier>PMID: 27063722</identifier><language>eng</language><publisher>Singapore: The Japan Society for Analytical Chemistry</publisher><subject>Analytical Chemistry ; Aptamers, Nucleotide - chemistry ; Biosensing Techniques ; Chemistry ; Cocaine - analysis ; cocaine detection ; Deoxyribonucleic acid ; electrochemical measurements ; Electrochemical Techniques ; Electrodes ; ferrocene conjugated DNA aptamer ; Heat treatment ; Hot Temperature ; Immobilization ; Mathematical analysis ; Narcotics ; Sensors ; Terminals</subject><ispartof>Analytical Sciences, 2016/04/10, Vol.32(4), pp.469-472</ispartof><rights>2016 by The Japan Society for Analytical Chemistry</rights><rights>The Japan Society for Analytical Chemistry 2016</rights><rights>Copyright Japan Science and Technology Agency 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c745t-88413066da4f5fe20ea71d0c7f52521d7f7bdf51bdb3607951dcf37d73f9b9753</citedby><cites>FETCH-LOGICAL-c745t-88413066da4f5fe20ea71d0c7f52521d7f7bdf51bdb3607951dcf37d73f9b9753</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,1876,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27063722$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ARIMOTO, Satoshi</creatorcontrib><creatorcontrib>SHIMONO, Ken</creatorcontrib><creatorcontrib>YASUKAWA, Tomoyuki</creatorcontrib><creatorcontrib>MIZUTANI, Fumio</creatorcontrib><creatorcontrib>YOSHIOKA, Toshihiko</creatorcontrib><title>Improvement of Electrochemical Response of Cocaine Sensors Based on DNA Aptamer by Heat Treatment</title><title>Analytical Sciences</title><addtitle>ANAL. SCI</addtitle><addtitle>Anal Sci</addtitle><description>We report on a biosensor for cocaine based on the conformation change of DNA aptamer by capturing the cocaine molecules. The oxidation current of ferrocene conjugated on the terminal end of aptamer immobilized on an Au electrode increased with increasing cocaine concentration. The sensor response has been improved by a simple heat treatment after immobilization, since the aggregates of DNA aptamer generated during the immobilization step could be dissociated and rearranged on the electrode.</description><subject>Analytical Chemistry</subject><subject>Aptamers, Nucleotide - chemistry</subject><subject>Biosensing Techniques</subject><subject>Chemistry</subject><subject>Cocaine - analysis</subject><subject>cocaine detection</subject><subject>Deoxyribonucleic acid</subject><subject>electrochemical measurements</subject><subject>Electrochemical Techniques</subject><subject>Electrodes</subject><subject>ferrocene conjugated DNA aptamer</subject><subject>Heat treatment</subject><subject>Hot Temperature</subject><subject>Immobilization</subject><subject>Mathematical analysis</subject><subject>Narcotics</subject><subject>Sensors</subject><subject>Terminals</subject><issn>0910-6340</issn><issn>1348-2246</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNqNkc9rFDEcxYModm29epSAFy-zze9MjutabaG0oO05ZDLftLPMTNZkVuh_b4ZdFxVED0kO7_Pe9xseQm8oWTJK1bkbXZ99t-RsKZR5hhaUi7piTKjnaEEMJZXigpygVzlvCKGsZuwlOmGaKK4ZWyB3NWxT_A4DjBOOAV_04KcU_SMMnXc9_gJ5G8cMs7aO3nUj4K8w5pgy_uAytDiO-OPNCq-2kxsg4eYJX4Kb8F0q95x6hl6EsiO8Pryn6P7Txd36srq-_Xy1Xl1XXgs5VXUtKCdKtU4EGYARcJq2xOsgmWS01UE3bZC0aRuuiDaStj5w3WoeTGO05Kfo_T63_OfbDvJkhy576Hs3QtxlS2sqTc3rcv6JaiOEqZU2_4HWRFJqKC_ouz_QTdyluZ-Z0oRpqebZyz3lU8w5QbDb1A0uPVlK7FypPVRqObOl0mJ4e4jdNQO0R_xnhwU43wO5SOMDpF_m_i1ytXds8uQe4Bjp0tT5Hn7DD56j5h9dsjDyHztQxBU</recordid><startdate>2016</startdate><enddate>2016</enddate><creator>ARIMOTO, Satoshi</creator><creator>SHIMONO, Ken</creator><creator>YASUKAWA, Tomoyuki</creator><creator>MIZUTANI, Fumio</creator><creator>YOSHIOKA, Toshihiko</creator><general>The Japan Society for Analytical Chemistry</general><general>Springer Nature Singapore</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SE</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>FR3</scope><scope>H8G</scope><scope>JG9</scope><scope>L7M</scope><scope>P64</scope><scope>7X8</scope><scope>7TM</scope></search><sort><creationdate>2016</creationdate><title>Improvement of Electrochemical Response of Cocaine Sensors Based on DNA Aptamer by Heat Treatment</title><author>ARIMOTO, Satoshi ; SHIMONO, Ken ; YASUKAWA, Tomoyuki ; MIZUTANI, Fumio ; YOSHIOKA, Toshihiko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c745t-88413066da4f5fe20ea71d0c7f52521d7f7bdf51bdb3607951dcf37d73f9b9753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Analytical Chemistry</topic><topic>Aptamers, Nucleotide - chemistry</topic><topic>Biosensing Techniques</topic><topic>Chemistry</topic><topic>Cocaine - analysis</topic><topic>cocaine detection</topic><topic>Deoxyribonucleic acid</topic><topic>electrochemical measurements</topic><topic>Electrochemical Techniques</topic><topic>Electrodes</topic><topic>ferrocene conjugated DNA aptamer</topic><topic>Heat treatment</topic><topic>Hot Temperature</topic><topic>Immobilization</topic><topic>Mathematical analysis</topic><topic>Narcotics</topic><topic>Sensors</topic><topic>Terminals</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ARIMOTO, Satoshi</creatorcontrib><creatorcontrib>SHIMONO, Ken</creatorcontrib><creatorcontrib>YASUKAWA, Tomoyuki</creatorcontrib><creatorcontrib>MIZUTANI, Fumio</creatorcontrib><creatorcontrib>YOSHIOKA, Toshihiko</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><jtitle>Analytical Sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ARIMOTO, Satoshi</au><au>SHIMONO, Ken</au><au>YASUKAWA, Tomoyuki</au><au>MIZUTANI, Fumio</au><au>YOSHIOKA, Toshihiko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improvement of Electrochemical Response of Cocaine Sensors Based on DNA Aptamer by Heat Treatment</atitle><jtitle>Analytical Sciences</jtitle><stitle>ANAL. SCI</stitle><addtitle>Anal Sci</addtitle><date>2016</date><risdate>2016</risdate><volume>32</volume><issue>4</issue><spage>469</spage><epage>472</epage><pages>469-472</pages><issn>0910-6340</issn><eissn>1348-2246</eissn><abstract>We report on a biosensor for cocaine based on the conformation change of DNA aptamer by capturing the cocaine molecules. The oxidation current of ferrocene conjugated on the terminal end of aptamer immobilized on an Au electrode increased with increasing cocaine concentration. The sensor response has been improved by a simple heat treatment after immobilization, since the aggregates of DNA aptamer generated during the immobilization step could be dissociated and rearranged on the electrode.</abstract><cop>Singapore</cop><pub>The Japan Society for Analytical Chemistry</pub><pmid>27063722</pmid><doi>10.2116/analsci.32.469</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0910-6340
ispartof Analytical Sciences, 2016/04/10, Vol.32(4), pp.469-472
issn 0910-6340
1348-2246
language eng
recordid cdi_proquest_miscellaneous_1815983898
source J-STAGE Free; Springer Nature
subjects Analytical Chemistry
Aptamers, Nucleotide - chemistry
Biosensing Techniques
Chemistry
Cocaine - analysis
cocaine detection
Deoxyribonucleic acid
electrochemical measurements
Electrochemical Techniques
Electrodes
ferrocene conjugated DNA aptamer
Heat treatment
Hot Temperature
Immobilization
Mathematical analysis
Narcotics
Sensors
Terminals
title Improvement of Electrochemical Response of Cocaine Sensors Based on DNA Aptamer by Heat Treatment
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T17%3A24%3A26IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Improvement%20of%20Electrochemical%20Response%20of%20Cocaine%20Sensors%20Based%20on%20DNA%20Aptamer%20by%20Heat%20Treatment&rft.jtitle=Analytical%20Sciences&rft.au=ARIMOTO,%20Satoshi&rft.date=2016&rft.volume=32&rft.issue=4&rft.spage=469&rft.epage=472&rft.pages=469-472&rft.issn=0910-6340&rft.eissn=1348-2246&rft_id=info:doi/10.2116/analsci.32.469&rft_dat=%3Cproquest_cross%3E1815983898%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c745t-88413066da4f5fe20ea71d0c7f52521d7f7bdf51bdb3607951dcf37d73f9b9753%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1787027568&rft_id=info:pmid/27063722&rfr_iscdi=true