Optimization of Physical and Nutritional Factors for Induced Production of Cellulase by Co-Culture Solid-State Bio-Processing of Corn Stover

Bio-conversion of cellulosic based biomass materials for cellulase production is one among the major increasing demand for various biotechnological applications. Therefore, the purpose of the present research work was to study the production and physical and nutritional parameters optimization for t...

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Bibliographic Details
Published in:WSEAS Transactions on Environment and Development 2013-10, Vol.9 (4)
Main Authors: Shahzadi, Tanzila, Ikram, Nadia, Rashid, Umer, Afroz, Amber, But, Hamama Islam, Anwar, Zahid, Irshad, Muhammad, Mahmood, Sajid
Format: Article
Language:English
Subjects:
Cellulase
Corn
Fermentation
Ganoderma lucidum
Optimization
Substrates
Synthesis
Trichoderma viride
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Summary:Bio-conversion of cellulosic based biomass materials for cellulase production is one among the major increasing demand for various biotechnological applications. Therefore, the purpose of the present research work was to study the production and physical and nutritional parameters optimization for the synthesis of cellulase from co-culture of Trichoderma viride and Ganoderma lucidum in solid state fermentation (SSF) using agro-industrial material corn stover as fungal growth supported substrates. Analysis profile showed that when the conditions of the SSF medium containing 15 g corn stover substrate (50% w/w moisture) inoculated with 6 mL of inoculum were optimal, the maximum productions of cellulase (466 + or - 5.6 U/mL) were recorded after 6 days of incubation at pH 6 and 35 [degrees]C. In conclusion, the present research findings will be supportive in the improvement of low cost system for hyper-production of carboxymethyl cellulase for industrial application.
ISSN:1790-5079
2224-3496