The adhesion of normal human dermal fibroblasts to the cyclopropylamine plasma polymers studied by holographic microscopy

The understanding of cell–surface interactions plays an important role for the biomaterials development and bioengineering. Although it is already known that amine groups increase the cell adhesion and proliferation, the influence of amine layers properties on cell viability is the subject of furthe...

Full description

Saved in:
Bibliographic Details
Published in:Surface & coatings technology 2016-06, Vol.295, p.70-77
Main Authors: Štrbková, L., Manakhov, A., Zajíčková, L., Stoica, A., Veselý, P., Chmelík, R.
Format: Article
Language:English
Subjects:
Amines
Cell adhesion
Coatings
Cyclopropylamine
Fibroblasts
Holographic microscopy
Microscopy
Monitoring
Plasma polymer
Quantitative phase imaging
Radio frequencies
Viability
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The understanding of cell–surface interactions plays an important role for the biomaterials development and bioengineering. Although it is already known that amine groups increase the cell adhesion and proliferation, the influence of amine layers properties on cell viability is the subject of further investigation. In this work, amine-rich coatings were prepared by low pressure plasma polymerization of cyclopropylamine using radio frequency (RF) capacitively coupled discharge. Normal human dermal fibroblasts were chosen for the monitoring of biological response to the properties of amine layers. As a superior technique for the label-free monitoring of the cell–surface interaction, coherence-controlled holographic microscopy (CCHM) was exploited. CCHM enables quantitative phase imaging. From such images, valuable morphological parameters of cells directly related to the cell dry mass can be extracted. Based on those parameters, viability of cells cultivated on the plasma-treated surfaces with different properties was studied and evaluated. According to the results, amine-rich films enhanced the conditions for the cell adhesion and proliferation. •Stable amine-rich thin films for the enhanced adhesion of normal human fibroblasts were prepared.•Living cells seeded on the amine surfaces were observed and analysed by Coherence-Controlled Holographic Microscopy.•Amine groups deposited by plasma polymerization of non-toxic cyclopropylamine enhanced the adhesion of observed cells.
ISSN:0257-8972
1879-3347
DOI:10.1016/j.surfcoat.2015.10.076