Zinc(II) and pyrophosphate selective fluorescence probe and its application to living cell imaging

A BODIPY-based fluorescent chemosensor 1 selectively detects Zn2+ by significant fluorescence enhancement. The binding ratio of the 1-Zn2+ complex was determined from a Job plot to be 1:1. The binding constant (Ka) of Zn2+ binding to chemosensor 1 was found to be 5.9×104M−1, with a detection limit o...

Full description

Saved in:
Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2015-02, Vol.207, p.563-570
Main Authors: Lin, Jia-Rong, Chu, Chien-Ju, Venkatesan, Parthiban, Wu, Shu-Pao
Format: Article
Language:English
Subjects:
Actuators
Binding
Cells (biology)
Chemoreceptors
Constants
Fluorescence
Imaging
Imaging agents
Pyrophosphate
Quenching
Sensors
Zn(II)
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A BODIPY-based fluorescent chemosensor 1 selectively detects Zn2+ by significant fluorescence enhancement. The binding ratio of the 1-Zn2+ complex was determined from a Job plot to be 1:1. The binding constant (Ka) of Zn2+ binding to chemosensor 1 was found to be 5.9×104M−1, with a detection limit of 0.59μM. Furthermore, the 1-Zn2+ complex can specifically detect pyrophosphate (PPi) through fluorescence quenching. The detection limit for detecting PPi was found to be approximately 17.5μM. Fluorescence microscopy imaging using RAW264.7 cells showed that chemosensor 1 can be used as an effective fluorescent probe for detecting Zn2+ and PPi in living cells.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2014.10.109