Extraction and purification of phytol from Abutilon indicum: cytotoxic and apoptotic activity

Medicinal plants possess a wide range of secondary metabolites and most of these secondary metabolites have their own importance in the field of medicine. Abutilon indicum is widely used as a medicinal plant in the Indian system of medicine. In the present study, we have evaluated the apoptosis indu...

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Bibliographic Details
Published in:RSC advances 2016-01, Vol.6 (54), p.48336-48345
Main Authors: Thakor, Parth, Mehta, Japan B., Patel, Ravi R., Patel, Disha D., Subramanian, Ramalingam B., Thakkar, Vasudev R.
Format: Article
Language:English
Subjects:
Apoptosis
Biocompatibility
Extraction
Medicine
Metabolites
Plants (organisms)
Purification
Two dimensional
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Summary:Medicinal plants possess a wide range of secondary metabolites and most of these secondary metabolites have their own importance in the field of medicine. Abutilon indicum is widely used as a medicinal plant in the Indian system of medicine. In the present study, we have evaluated the apoptosis inducing ability of leaf extract of A. indicum . Hydromethanolic leaf extract showed cytotoxicity on Schizosaccharomyces pombe cells by affecting the growth and viability. Phytochemical analysis of the extract revealed the presence of secondary metabolites. A 2D TLC method was used for the separation of the active cytotoxic compound. A HPTLC method was used for the quantification of the bioactive compound. A GC-HRMS method was employed for the identification of the active principle as phytol, which was found to be responsible for inducing ROS mediated apoptosis in S. pombe .
ISSN:2046-2069
2046-2069
DOI:10.1039/C5RA24464A