Determination and pharmacokinetic study of four lignans in rat plasma after oral administration of an extract of Valeriana amurensis by ultra-high performance liquid chromatography with tandem mass spectrometry

• Published in: Journal of separation science 2016-05, Vol.39 (10), p.1825-1833
• Main Authors: Xue, Juan, Mi, Yingying, Wang, Zhibin, Sun, Yichun, Wu, Qiong, Wang, Changfu, Zhang, Hongwei, Yang, Xin, Kuang, Haixue, Wang, Qiuhong
• Format: Article
• Language: English
• Subjects: Administration, Oral; Animals; Chromatography; Chromatography, High Pressure Liquid; Ionization; Ions; Lignan; Lignans - blood; Lignans - chemistry; Lignans - pharmacokinetics; Liquid chromatography; Male; Mass spectrometers; Mass spectrometry; Molecular Structure; Monitoring; Pharmacokinetics; Pharmacology; Plant Extracts - administration & dosage; Plant Extracts - blood; Plant Extracts - pharmacokinetics; Quadrupoles; Rats; Rats, Sprague-Dawley; Rodents; Scientific imaging; Tandem Mass Spectrometry; Valerian - chemistry; Valeriana amurensis
• ISSN: 1615-9306; 1615-9314
• DOI: 10.1002/jssc.201600038

A selective and sensitive ultra‐high performance liquid chromatography with tandem mass spectrometry method was developed and validated for the determination and pharmacokinetic study of (+)‐8‐hydroxypinoresinol‐4’‐O‐β ‐D‐glucopyranoside, prinsepiol‐4‐O‐β‐D‐glucopyranoside, (+)‐pinoresinol‐4,4’‐di‐O‐β‐D‐glucopyranoside, and (−)‐massoniresinol 3α‐O‐β‐D‐glucopyranoside in rat plasma after the oral administration of a Valeriana amurensis extract. The analytes and ethyl 4‐hydroxybenzoate (internal standard) were separated on a Waters ACQUITY UPLC HSS T3 chromatographic column. The detection was performed on a triple quadrupole tandem mass spectrometer in multiple reaction monitoring mode using an electrospray ionization source operating in negative ionization mode. The linear ranges (ng/mL) of the standard curves were 0.39–154.00, 0.62–244.70, 0.50–198.60, and 0.34–134.50 for (+)‐8‐hydroxypinoresinol‐4’‐O‐β‐D‐glucopyranoside, prinsepiol‐4‐O‐β‐D‐glucopyranoside, (+)‐pinoresinol‐4,4’‐di‐O‐β‐D‐glucopyranoside, and (−)‐massoniresinol 3α‐O‐β‐D‐glucopyranoside, respectively. The inter‐ and intra‐day precisions were less than 11.0%, the accuracies were between −5.9 and 7.7%, and the extraction recoveries of the four analytes were > 81.2% from rat plasma. The method was successfully applied to a pharmacokinetic study of the four analytes after oral administration of a Valeriana amurensis extract to rats. The developed method has the potential for pharmacokinetic analysis and to provide additional information in the clinical application of Valeriana amurensis.