Mouthguard biosensor with telemetry system for monitoring of saliva glucose: A novel cavitas sensor

• Published in: Biosensors & bioelectronics 2016-10, Vol.84, p.106-111
• Main Authors: Arakawa, Takahiro, Kuroki, Yusuke, Nitta, Hiroki, Chouhan, Prem, Toma, Koji, Sawada, Shin-ichi, Takeuchi, Shuhei, Sekita, Toshiaki, Akiyoshi, Kazunari, Minakuchi, Shunsuke, Mitsubayashi, Kohji
• Format: Article
• Language: English
• Subjects: Biosensing Techniques - instrumentation; Biosensor; Biosensors; Electrodes; Enzymes, Immobilized - chemistry; Equipment Design; Glucose; Glucose - analysis; Glucose oxidase; Glucose Oxidase - chemistry; Human; Humans; Monitoring; Monitoring, Physiologic; Mouthguard; Mouthguards; Oral cavity; Polyethylene Glycols - chemistry; Polyethylene Terephthalates - chemistry; Saliva; Saliva - chemistry; Saliva glucose; Sensors; Telemetry
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2015.12.014

We develop detachable “Cavitas sensors” to apply to the human oral cavity for non-invasive monitoring of saliva glucose. A salivary biosensor incorporating Pt and Ag/AgCl electrodes on a mouthguard support with an enzyme membrane is developed and tested. Electrodes are formed on the polyethylene terephthalate glycol (PETG) surface of the mouthguard. The Pt working electrode is coated with a glucose oxidase (GOD) membrane. The biosensor seamlessly is integrated with a glucose sensor and a wireless measurement system. When investigating in-vitro performance, the biosensor exhibits a robust relationship between output current and glucose concentration. In artificial saliva composed of salts and proteins, the glucose sensor is capable of highly sensitive detection over a range of 5–1000µmol/L of glucose, which encompasses the range of glucose concentrations found in human saliva. We demonstrate the ability of the sensor and wireless communication module to monitor saliva glucose in a phantom jaw imitating the structure of the human oral cavity. Stable and long-term real-time monitoring (exceeding 5h) with the telemetry system is achieved. The mouthguard biosensor will be useful as a novel method for real-time non-invasive saliva glucose monitoring for better management of dental patients. •We developed a mouthguard biosensor for non-invasive monitoring of saliva glucose.•The biosensor is formed on a mouthguard surface made of dental PETG.•High-sensitive detection including the range of human salivary glucose was achieved.•Highly sensitive and selective monitoring of glucose in artificial saliva was demonstrated.•Monitoring of glucose concentration was demonstrated using a phantom jaw.