Dopamine biosensor based on surface functionalized nanostructured nickel oxide platform

• Published in: Biosensors & bioelectronics 2016-10, Vol.84, p.72-81
• Main Authors: Roychoudhury, Appan, Basu, Suddhasatwa, Jha, Sandeep Kumar
• Format: Article
• Language: English
• Subjects: Agaricus - enzymology; Animals; Biosensing Techniques - methods; Biosensors; Cattle; Dopamine; Dopamine - blood; Dopamine biosensor; Electrochemical sensor; Electrochemical Techniques - methods; Enzymes; Enzymes, Immobilized - chemistry; Indium tin oxide; Limit of Detection; Monophenol Monooxygenase - chemistry; Nanoparticles; Nanoparticles - chemistry; Nanoparticles - ultrastructure; Neurochemical detection; Nickel - chemistry; Nickel oxides; NiO nanoparticles; Polyethylene Terephthalates - chemistry; Tin Compounds - chemistry; Transmission electron microscopy; Tyrosinase
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2015.11.061

A dopamine biosensor has been developed using nickel oxide nanoparticles (NPs) and tyrosinase enzyme conjugate. Nickel oxide (NiO) NPs were synthesized by sol–gel method using anionic surfactant, sodium dodecyl sulphate (SDS), as template to control the size of synthesized nanoparticles. The structural and morphological studies of the prepared NPs were carried out using X-ray diffraction (XRD), transmission electron microscopy (TEM) and dynamic light scattering (DLS) techniques. Afterwards, tyrosinase enzyme molecules were adsorbed on NiO NPs surface and enzyme coated NPs were deposited on indium tin oxide (ITO) coated flexible polyethylene terephthalate (PET) substrate by solution casting method. The formation of enzyme–NPs conjugate was investigated by atomic force microscopy (AFM) and Fourier transform infrared spectroscopy (FTIR) techniques and used in selective detection and estimation of neurochemical dopamine by electrochemical method. The fabricated Tyrosinase/NiO/ITO electrode exhibits high sensitivity of 60.2nA/µM in linear detection range (2–100μM) with a detection limit of 1.038μM. The proposed sensor had a response time of 45s, long shelf life (45 days) with good reproducibility and selectivity in presence of interfering substances and was validated with real samples. The tyrosinase enzyme functionalized NiO platform has good bio-sensing efficacy and can be used in detection of other catecholamines and phenolic neurochemicals. •Dopamine biosensor was developed using tyrosinase/NiO deposited on ITO coated PET.•Biosensor exhibited 0.0602 µA/µM sensitivity, LOD of 1.038 μM and range 2-500 μM.•Sensor shows high selectivity in interfering ascorbic acid and uric acid (RSD 5-15%).•Biosensor had response time of 45 s, excellent reusability and 45 days of shelf life.