Graphene based aptasensor for glycated albumin in diabetes mellitus diagnosis and monitoring

We selected and modified DNA aptamers specifically bound glycated human serum albumin (GHSA), which is an intermediate marker for diabetes mellitus. Our aptamer truncation study indicated that the hairpin-loop structure with 23 nucleotides length containing triple G-C hairpins and 15-nucleotide loop...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2016-08, Vol.82, p.140-145
Main Authors: Apiwat, Chayachon, Luksirikul, Patraporn, Kankla, Pacharapon, Pongprayoon, Prapasiri, Treerattrakoon, Kiatnida, Paiboonsukwong, Kittiphong, Fucharoen, Suthat, Dharakul, Tararaj, Japrung, Deanpen
Format: Article
Language:English
Subjects:
Aptamer
Aptamers, Nucleotide - chemistry
Aptasensor
Base Sequence
Binding
Biosensing Techniques - methods
Biosensors
Carbocyanines - chemistry
Diabetes mellitus
Diabetes Mellitus - blood
Diabetes Mellitus - diagnosis
Diagnosis
Fluorescence
Fluorescent Dyes - chemistry
Glycated human serum albumin
Graphene
Graphene oxide
Graphite - chemistry
Humans
Limit of Detection
Monitoring
Oxides - chemistry
Serum albumin
Serum Albumin - analysis
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Summary:We selected and modified DNA aptamers specifically bound glycated human serum albumin (GHSA), which is an intermediate marker for diabetes mellitus. Our aptamer truncation study indicated that the hairpin-loop structure with 23 nucleotides length containing triple G-C hairpins and 15-nucleotide loop, plays an important role in GHSA binding. Fluorescent quenching graphene oxide (GO) and Cy5-labeled G8 aptamer were used in this study to develop simple and sensitive graphene based aptasensor for GHSA detection. The limit of detection (LOD) of our aptasensor was 50 μg/mL, which was lower than other existing methods. In addition, with the nuclease resistance system, our GHSA detection platform could also be used in clinical samples. Importantly, our approach could significantly reveal the higher levels of GHSA concentrations in diabetes than normal serums. These indicate that our aptasensor has a potential for diagnosis and monitoring of diabetes mellitus. •Aptamers bound GHSA, which is a marker for diabetes mellitus, were selected.•A simple and sensitive GHSA aptasensor platform was developed.•High GHSA concentrations from diabetic serums were observed.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2016.04.015