Acid tolerance mediated by membrane ATPases in Lactobacillus acidophilus

The acid tolerance response in Lactobacillus acidophilus CRL 639, induced at pH 4.2 for 15 min, is mediated by the cell membrane F^sub 1^-F^sub 0^ ATPase. The specific activity of the enzyme was induced 1.6-fold after acid adaptation compared to a non-adapted control. The ATPase was optimal at pH 6...

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Bibliographic Details
Published in:Biotechnology letters 2001-05, Vol.23 (10), p.777-780
Main Authors: LORCA, G. L, FONT DE VALDEZ, G
Format: Article
Language:English
Subjects:
Action of physical and chemical agents on bacteria
Bacteriology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Lactobacillus acidophilus
Microbiology
Proteins
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Summary:The acid tolerance response in Lactobacillus acidophilus CRL 639, induced at pH 4.2 for 15 min, is mediated by the cell membrane F^sub 1^-F^sub 0^ ATPase. The specific activity of the enzyme was induced 1.6-fold after acid adaptation compared to a non-adapted control. The ATPase was optimal at pH 6 with a K^sub m^=0.8 mM and a V^sub max^=100 mM.[PUBLICATION ABSTRACT]
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1010346131918