Effects of fusaric acid treatment on the protocorm-like bodies of Dendrobium sonia-28

Dendrobium sonia-28 is a popular orchid hybrid due to its flowering recurrence and dense inflorescences. Unfortunately, it is being decimated by fungal diseases, especially those caused by Fusarium proliferatum . In this study, selection of F. proliferatum -tolerant protocorm-like bodies (PLBs) was...

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Bibliographic Details
Published in:Protoplasma 2016-09, Vol.253 (5), p.1373-1383
Main Authors: Dehgahi, Raheleh, Zakaria, Latiffah, Mohamad, Azhar, Joniyas, Alireza, Subramaniam, Sreeramanan
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Cell Biology
Cell Wall - pathology
Dendrobium - embryology
Dendrobium - microbiology
Dendrobium - ultrastructure
Fusaric Acid - pharmacology
Fusarium - pathogenicity
Life Sciences
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Original Article
Plant Diseases - microbiology
Plant Sciences
Plant Shoots - growth & development
Plant Shoots - ultrastructure
Random Amplified Polymorphic DNA Technique
Zoology
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Summary:Dendrobium sonia-28 is a popular orchid hybrid due to its flowering recurrence and dense inflorescences. Unfortunately, it is being decimated by fungal diseases, especially those caused by Fusarium proliferatum . In this study, selection of F. proliferatum -tolerant protocorm-like bodies (PLBs) was carried out by assessing the effects of differing concentrations of fusaric acid (FA). PLBs were cultured on Murashige and Skoog (MS) medium supplemented with 0.05 to 0.2 millimolar (mM) concentrations of FA. Higher concentrations of FA increased mortality of PLBs and reduced their growth. The survival rate for 0.05 mM FA was 20 % but only 1 % at the highest dose of 0.2 mM. Additionally, two different size ranges of PLBs were investigated, and growth increased more at lower FA concentrations for larger PLBs, whilst the growth rate of smaller PLBs was inhibited at an FA concentration of 0.2 mM. Histological examination using transmission electron microscopy (TEM) and scanning electron microscopy (SEM) analyses disclosed severe cell wall and organelle damage, as well as stomatal closure in PLBs treated with the high FA concentrations. Reductions in plantlet growth were much greater at the highest concentrations of FA. Some randomly amplified polymorphic DNA (RAPD) markers clearly discriminated between selected and non-selected variants of Dendrobium sonia-28, showing different banding patterns for each FA concentration and specific bands for selected and control plants.
ISSN:0033-183X
1615-6102
DOI:10.1007/s00709-015-0895-1