Tumour suppressive function of HUWE1 in thyroid cancer

HUWE1 (the HECT, UBA, and WWE domain-containing protein 1) is an ubiquitin E3 ligase which plays an important role in coordinating diverse cellular processes. It has been found to be dysregulated in various cancer type and its functions in tumorigenesis remain controversial. The potential tumour sup...

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Bibliographic Details
Published in:Journal of biosciences 2016-09, Vol.41 (3), p.395-405
Main Authors: Ma, Weiyuan, Zhao, Pengxin, Zang, Leilei, Zhang, Kaili, Liao, Haiying, Hu, Zhigang
Format: Article
Language:English
Subjects:
Animals
Bioassays
Biomarkers, Tumor - biosynthesis
Biomarkers, Tumor - genetics
Biomedical and Life Sciences
Biomedicine
Carcinogenesis - genetics
Cell Biology
Cell Line, Tumor
Cell Movement - genetics
Cell Proliferation - genetics
Cellular biology
Gene Expression Regulation, Neoplastic
Gene Knockdown Techniques
Humans
Life Sciences
Mice
Microbiology
Neoplasm Invasiveness - genetics
Plant Sciences
Thyroid
Thyroid cancer
Thyroid Neoplasms - genetics
Thyroid Neoplasms - pathology
Tumor Suppressor Protein p53 - genetics
Tumors
Ubiquitin-Protein Ligases - biosynthesis
Ubiquitin-Protein Ligases - genetics
Xenograft Model Antitumor Assays
Zoology
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Summary:HUWE1 (the HECT, UBA, and WWE domain-containing protein 1) is an ubiquitin E3 ligase which plays an important role in coordinating diverse cellular processes. It has been found to be dysregulated in various cancer type and its functions in tumorigenesis remain controversial. The potential tumour suppressive role of HUWE1 in thyroid cancer development was investigated by knocking down HUWE1 in three authentic thyroid cancer cell lines, WRO, FTC133 and BCPAP, followed by various functional assays, including cell proliferation, scratch wound healing and invasion assays. Xenograft experiment was performed to examine in vivo tumour suppressive properties of HUWE1. Small-interfering RNA mediated knockdown of HUWE1 promoted cell proliferation, cell migration and invasion in thyroid cancer cells. Overexpression of HUWE1 conferred partial sensitivity to chemo drugs interfering with DNA replication in these cells. Moreover, HUWE1 was found to be down-regulated in human thyroid cancer tissues compared with matched normal thyroid tissues. In addition, overexpression of HUWE1 significantly inhibited tumour growth in vivo using xenograft mouse models. Mechanistic investigation revealed that HUWE1 can regulate p53 protein level through its stabilization. HUWE1 functions as a tumour suppressor in thyroid cancer progression, which may represent a novel therapeutic target for prevention or intervention of thyroid cancer.
ISSN:0250-5991
0973-7138
DOI:10.1007/s12038-016-9623-z