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Tumour suppressive function of HUWE1 in thyroid cancer
HUWE1 (the HECT, UBA, and WWE domain-containing protein 1) is an ubiquitin E3 ligase which plays an important role in coordinating diverse cellular processes. It has been found to be dysregulated in various cancer type and its functions in tumorigenesis remain controversial. The potential tumour sup...
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| Published in: | Journal of biosciences 2016-09, Vol.41 (3), p.395-405 |
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| container_end_page | 405 |
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| container_title | Journal of biosciences |
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| creator | Ma, Weiyuan Zhao, Pengxin Zang, Leilei Zhang, Kaili Liao, Haiying Hu, Zhigang |
| description | HUWE1 (the HECT, UBA, and WWE domain-containing protein 1) is an ubiquitin E3 ligase which plays an important role in coordinating diverse cellular processes. It has been found to be dysregulated in various cancer type and its functions in tumorigenesis remain controversial. The potential tumour suppressive role of HUWE1 in thyroid cancer development was investigated by knocking down HUWE1 in three authentic thyroid cancer cell lines, WRO, FTC133 and BCPAP, followed by various functional assays, including cell proliferation, scratch wound healing and invasion assays. Xenograft experiment was performed to examine
in vivo
tumour suppressive properties of HUWE1. Small-interfering RNA mediated knockdown of HUWE1 promoted cell proliferation, cell migration and invasion in thyroid cancer cells. Overexpression of HUWE1 conferred partial sensitivity to chemo drugs interfering with DNA replication in these cells. Moreover, HUWE1 was found to be down-regulated in human thyroid cancer tissues compared with matched normal thyroid tissues. In addition, overexpression of HUWE1 significantly inhibited tumour growth
in vivo
using xenograft mouse models. Mechanistic investigation revealed that HUWE1 can regulate p53 protein level through its stabilization. HUWE1 functions as a tumour suppressor in thyroid cancer progression, which may represent a novel therapeutic target for prevention or intervention of thyroid cancer. |
| doi_str_mv | 10.1007/s12038-016-9623-z |
| format | article |
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in vivo
tumour suppressive properties of HUWE1. Small-interfering RNA mediated knockdown of HUWE1 promoted cell proliferation, cell migration and invasion in thyroid cancer cells. Overexpression of HUWE1 conferred partial sensitivity to chemo drugs interfering with DNA replication in these cells. Moreover, HUWE1 was found to be down-regulated in human thyroid cancer tissues compared with matched normal thyroid tissues. In addition, overexpression of HUWE1 significantly inhibited tumour growth
in vivo
using xenograft mouse models. Mechanistic investigation revealed that HUWE1 can regulate p53 protein level through its stabilization. HUWE1 functions as a tumour suppressor in thyroid cancer progression, which may represent a novel therapeutic target for prevention or intervention of thyroid cancer.</description><identifier>ISSN: 0250-5991</identifier><identifier>EISSN: 0973-7138</identifier><identifier>DOI: 10.1007/s12038-016-9623-z</identifier><identifier>PMID: 27581931</identifier><language>eng</language><publisher>New Delhi: Springer India</publisher><subject>Animals ; Bioassays ; Biomarkers, Tumor - biosynthesis ; Biomarkers, Tumor - genetics ; Biomedical and Life Sciences ; Biomedicine ; Carcinogenesis - genetics ; Cell Biology ; Cell Line, Tumor ; Cell Movement - genetics ; Cell Proliferation - genetics ; Cellular biology ; Gene Expression Regulation, Neoplastic ; Gene Knockdown Techniques ; Humans ; Life Sciences ; Mice ; Microbiology ; Neoplasm Invasiveness - genetics ; Plant Sciences ; Thyroid ; Thyroid cancer ; Thyroid Neoplasms - genetics ; Thyroid Neoplasms - pathology ; Tumor Suppressor Protein p53 - genetics ; Tumors ; Ubiquitin-Protein Ligases - biosynthesis ; Ubiquitin-Protein Ligases - genetics ; Xenograft Model Antitumor Assays ; Zoology</subject><ispartof>Journal of biosciences, 2016-09, Vol.41 (3), p.395-405</ispartof><rights>Indian Academy of Sciences 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-25077b84a05c07390ad3cba9078096bef18116d7f456e8098dc79a884124b2c13</citedby><cites>FETCH-LOGICAL-c405t-25077b84a05c07390ad3cba9078096bef18116d7f456e8098dc79a884124b2c13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27581931$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ma, Weiyuan</creatorcontrib><creatorcontrib>Zhao, Pengxin</creatorcontrib><creatorcontrib>Zang, Leilei</creatorcontrib><creatorcontrib>Zhang, Kaili</creatorcontrib><creatorcontrib>Liao, Haiying</creatorcontrib><creatorcontrib>Hu, Zhigang</creatorcontrib><title>Tumour suppressive function of HUWE1 in thyroid cancer</title><title>Journal of biosciences</title><addtitle>J Biosci</addtitle><addtitle>J Biosci</addtitle><description>HUWE1 (the HECT, UBA, and WWE domain-containing protein 1) is an ubiquitin E3 ligase which plays an important role in coordinating diverse cellular processes. It has been found to be dysregulated in various cancer type and its functions in tumorigenesis remain controversial. The potential tumour suppressive role of HUWE1 in thyroid cancer development was investigated by knocking down HUWE1 in three authentic thyroid cancer cell lines, WRO, FTC133 and BCPAP, followed by various functional assays, including cell proliferation, scratch wound healing and invasion assays. Xenograft experiment was performed to examine
in vivo
tumour suppressive properties of HUWE1. Small-interfering RNA mediated knockdown of HUWE1 promoted cell proliferation, cell migration and invasion in thyroid cancer cells. Overexpression of HUWE1 conferred partial sensitivity to chemo drugs interfering with DNA replication in these cells. Moreover, HUWE1 was found to be down-regulated in human thyroid cancer tissues compared with matched normal thyroid tissues. In addition, overexpression of HUWE1 significantly inhibited tumour growth
in vivo
using xenograft mouse models. Mechanistic investigation revealed that HUWE1 can regulate p53 protein level through its stabilization. HUWE1 functions as a tumour suppressor in thyroid cancer progression, which may represent a novel therapeutic target for prevention or intervention of thyroid cancer.</description><subject>Animals</subject><subject>Bioassays</subject><subject>Biomarkers, Tumor - biosynthesis</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Carcinogenesis - genetics</subject><subject>Cell Biology</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement - genetics</subject><subject>Cell Proliferation - genetics</subject><subject>Cellular biology</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Gene Knockdown Techniques</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Mice</subject><subject>Microbiology</subject><subject>Neoplasm Invasiveness - genetics</subject><subject>Plant Sciences</subject><subject>Thyroid</subject><subject>Thyroid cancer</subject><subject>Thyroid Neoplasms - genetics</subject><subject>Thyroid Neoplasms - pathology</subject><subject>Tumor Suppressor Protein p53 - genetics</subject><subject>Tumors</subject><subject>Ubiquitin-Protein Ligases - biosynthesis</subject><subject>Ubiquitin-Protein Ligases - genetics</subject><subject>Xenograft Model Antitumor Assays</subject><subject>Zoology</subject><issn>0250-5991</issn><issn>0973-7138</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNqNkE9LwzAYh4Mobk4_gBcpePFSfd-kzZ-jjOmEgZcNj6FNU-1Y25mswvbpzegUEQQPISF5fr-XPIRcItwigLjzSIHJGJDHilMW747IEJRgsUAmj8OZphCnSuGAnHm_BECVMDglAypSiYrhkPB5V7edi3y3XjvrffVho7JrzKZqm6gto-niZYJR1USbt61rqyIyWWOsOycnZbby9uKwj8jiYTIfT-PZ8-PT-H4WmwTSTRzmC5HLJIPUgGAKsoKZPFMgJCie2xIlIi9EmaTchitZGKEyKROkSU4NshG56XvXrn3vrN_ouvLGrlZZY9vO65DnnHGawH_QlCsRVkCvf6HL4KAJH9lTmDIqWRIo7CnjWu-dLfXaVXXmthpB7_3r3r8O_vXev96FzNWhuctrW3wnvoQHgPaAD0_Nq3U_Rv_Z-gnwF41P</recordid><startdate>20160901</startdate><enddate>20160901</enddate><creator>Ma, Weiyuan</creator><creator>Zhao, Pengxin</creator><creator>Zang, Leilei</creator><creator>Zhang, Kaili</creator><creator>Liao, Haiying</creator><creator>Hu, Zhigang</creator><general>Springer India</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H99</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L.F</scope><scope>L.G</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7QO</scope><scope>7X8</scope></search><sort><creationdate>20160901</creationdate><title>Tumour suppressive function of HUWE1 in thyroid cancer</title><author>Ma, Weiyuan ; Zhao, Pengxin ; Zang, Leilei ; Zhang, Kaili ; Liao, Haiying ; Hu, Zhigang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-25077b84a05c07390ad3cba9078096bef18116d7f456e8098dc79a884124b2c13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Bioassays</topic><topic>Biomarkers, Tumor - 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Academic</collection><jtitle>Journal of biosciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ma, Weiyuan</au><au>Zhao, Pengxin</au><au>Zang, Leilei</au><au>Zhang, Kaili</au><au>Liao, Haiying</au><au>Hu, Zhigang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tumour suppressive function of HUWE1 in thyroid cancer</atitle><jtitle>Journal of biosciences</jtitle><stitle>J Biosci</stitle><addtitle>J Biosci</addtitle><date>2016-09-01</date><risdate>2016</risdate><volume>41</volume><issue>3</issue><spage>395</spage><epage>405</epage><pages>395-405</pages><issn>0250-5991</issn><eissn>0973-7138</eissn><abstract>HUWE1 (the HECT, UBA, and WWE domain-containing protein 1) is an ubiquitin E3 ligase which plays an important role in coordinating diverse cellular processes. It has been found to be dysregulated in various cancer type and its functions in tumorigenesis remain controversial. The potential tumour suppressive role of HUWE1 in thyroid cancer development was investigated by knocking down HUWE1 in three authentic thyroid cancer cell lines, WRO, FTC133 and BCPAP, followed by various functional assays, including cell proliferation, scratch wound healing and invasion assays. Xenograft experiment was performed to examine
in vivo
tumour suppressive properties of HUWE1. Small-interfering RNA mediated knockdown of HUWE1 promoted cell proliferation, cell migration and invasion in thyroid cancer cells. Overexpression of HUWE1 conferred partial sensitivity to chemo drugs interfering with DNA replication in these cells. Moreover, HUWE1 was found to be down-regulated in human thyroid cancer tissues compared with matched normal thyroid tissues. In addition, overexpression of HUWE1 significantly inhibited tumour growth
in vivo
using xenograft mouse models. Mechanistic investigation revealed that HUWE1 can regulate p53 protein level through its stabilization. HUWE1 functions as a tumour suppressor in thyroid cancer progression, which may represent a novel therapeutic target for prevention or intervention of thyroid cancer.</abstract><cop>New Delhi</cop><pub>Springer India</pub><pmid>27581931</pmid><doi>10.1007/s12038-016-9623-z</doi><tpages>11</tpages></addata></record> |
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| subjects | Animals Bioassays Biomarkers, Tumor - biosynthesis Biomarkers, Tumor - genetics Biomedical and Life Sciences Biomedicine Carcinogenesis - genetics Cell Biology Cell Line, Tumor Cell Movement - genetics Cell Proliferation - genetics Cellular biology Gene Expression Regulation, Neoplastic Gene Knockdown Techniques Humans Life Sciences Mice Microbiology Neoplasm Invasiveness - genetics Plant Sciences Thyroid Thyroid cancer Thyroid Neoplasms - genetics Thyroid Neoplasms - pathology Tumor Suppressor Protein p53 - genetics Tumors Ubiquitin-Protein Ligases - biosynthesis Ubiquitin-Protein Ligases - genetics Xenograft Model Antitumor Assays Zoology |
| title | Tumour suppressive function of HUWE1 in thyroid cancer |
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