The absence of 25-hydroxyvitamin D3-1α-hydroxylase potentiates the suppression of EAE in mice by ultraviolet light

• Published in: The Journal of steroid biochemistry and molecular biology 2016-10, Vol.163, p.98-102
• Main Authors: Wang, Yanping, Marling, Steve J., Martino, Victoria M., Prahl, Jean M., Deluca, Hector F.
• Format: Article
• Language: English
• Subjects: 25-Hydroxyvitamin D3 1-alpha-Hydroxylase - deficiency; 25-Hydroxyvitamin D3 1-alpha-Hydroxylase - genetics; Animals; Calcitriol - metabolism; Chemokine CCL5 - genetics; Chemokine CCL5 - metabolism; CYP27B1 knockout mice; Encephalomyelitis, Autoimmune, Experimental - genetics; Encephalomyelitis, Autoimmune, Experimental - metabolism; Encephalomyelitis, Autoimmune, Experimental - pathology; Encephalomyelitis, Autoimmune, Experimental - therapy; Experimental autoimmune encephalomyelitis; Female; Gene Expression Regulation; Interferon-gamma - genetics; Interferon-gamma - metabolism; Interleukin-10 - genetics; Interleukin-10 - metabolism; Mice; Mice, Knockout; Multiple sclerosis; RNA, Messenger - genetics; RNA, Messenger - metabolism; Ultraviolet Rays; Ultraviolet Therapy; UVB
• ISSN: 0960-0760; 1879-1220
• DOI: 10.1016/j.jsbmb.2016.04.010

•UV light completely blocks EAE in CYP27B1 KO mice.•UV light suppresses expression of cytokines IFN-γ, IL-10 and CCL-5 in spinal cord in EAE susceptible mice.•1,25-OH-D3 reduces effectiveness of UV light in suppressing EAE. Ultraviolet B (UVB) light suppresses the development of multiple sclerosis (MS) in patients and experimental autoimmune encephalomyelitis (EAE) in mice. Although vitamin D3 is produced by ultraviolet light, the suppression of EAE by narrow band UVB (NBUVB) is independent of vitamin D3. However, it is possible that the NBUVB suppression of EAE can be further influenced by 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3). We used NBUVB lamps (10KJ/m2) to irradiate both wild type (WT) and 1α-hydroxylase knockout mice (CYP27B1 KO) that were then induced to develop EAE. There was a complete elimination of EAE development by NBUVB in the KO mice. On the other hand, the NBUVB treatment of WT mice reduced but did not eliminate the severity or incidence of EAE. This suggests that the presence of 1,25-dihydroxyvitamin D3 actually counteracts the suppressive effect of NBUVB. In support of this concept, cytokines (IFN-γ, IL-10) and chemokine (CCL-5) mRNA in spinal cord were reduced in wild type or eliminated in the KO mice by the NBUVB. Cytokine mRNA levels in the spinal cord correlated with clinical scores in both WT and KO mice.