Effect of actinobacteria agent inoculation methods on cellulose degradation during composting based on redundancy analysis

• Published in: Bioresource technology 2016-11, Vol.219, p.196-203
• Main Authors: Zhao, Yue, Lu, Qian, Wei, Yuquan, Cui, Hongyang, Zhang, Xu, Wang, Xueqin, Shan, Si, Wei, Zimin
• Format: Article
• Language: English
• Subjects: Actinobacteria; Carbohydrate Metabolism; Cellulose; Cellulose degradation; Composting; Denaturing gradient gel electrophoresis (DGGE); Different stages inoculation; Manure - microbiology; Redundancy analysis (RDA); Refuse Disposal - methods; Soil; Soil Microbiology
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2016.07.117

[Display omitted] •Assess the degradation of cellulose and organic matter during different composting.•Compare the structure of actinobacteria community in different treatments.•Predict the degradation effect of different treatments based on kinetics models.•An analytical method for appropriate inoculation and composting time was proposed. In this study, actinobacteria agent including Streptomyces sp. and Micromonospora sp. were inoculated during chicken manure composting by different inoculation methods. The effect of different treatments on cellulose degradation and the relationship between inoculants and indigenous actinobacteria were investigated during composting. The results showed that inoculation in different stages of composting all improved the actinobacteria community diversity particularly in the cooling stage of composting (M3). Moreover, inoculation could distinctly accelerate the degradation of organic matters (OM) especially celluloses. Redundancy analysis indicated that the correlation between indigenous actinobacteria and degradation of OM and cellulose were regulated by inoculants and there were significant differences between different inoculation methods. Furthermore, synergy between indigenous actinobacteria and inoculants for degradation of OM and cellulose in M3 was better than other treatments. Conclusively, we suggested an inoculation method to regulate the indigenous actinobacteria based on the relationship between inoculants and indigenous actinobacteria and degradation content.