Tiagabine treatment and DNA damage in rat astrocytes: an in vitro study by comet assay

We studied in vitro the effects of Tiagabine on genomic DNA of cortical rat astrocytes. To evaluate DNA damage, we used a relatively simple technique called Single Cell Gel Electrophoresis or Comet assay. Tiagabine was dissolved in culture medium and added at concentration of 1, 10, 20 and 50 μg/ml...

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Bibliographic Details
Published in:Neuroscience letters 2001-06, Vol.306 (1), p.17-20
Main Authors: Cardile, Venera, Palumbo, Maddalena, Renis, Marcella, Pavone, Antonino, Maci, Tiziana, Perciavalle, Vincenzo
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn
Anticonvulsants. Antiepileptics. Antiparkinson agents
Antiepileptic drugs
Astrocytes - drug effects
Astrocytes - metabolism
Astrocytes - pathology
Biological and medical sciences
Cells, Cultured - drug effects
Cells, Cultured - metabolism
Cells, Cultured - pathology
Cerebral Cortex - drug effects
Cerebral Cortex - metabolism
Cerebral Cortex - physiopathology
Comet assay
Comet Assay - methods
DNA damage
DNA Damage - drug effects
DNA Damage - physiology
Dose-Response Relationship, Drug
Epilepsy - drug therapy
GABA Agonists - toxicity
Genome
Glial cells
Glial Fibrillary Acidic Protein - metabolism
Medical sciences
Nerve Degeneration - chemically induced
Nerve Degeneration - metabolism
Nerve Degeneration - physiopathology
Neuropharmacology
Nipecotic Acids - toxicity
Pharmacology. Drug treatments
Rat
Rats
Tiagabine
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Summary:We studied in vitro the effects of Tiagabine on genomic DNA of cortical rat astrocytes. To evaluate DNA damage, we used a relatively simple technique called Single Cell Gel Electrophoresis or Comet assay. Tiagabine was dissolved in culture medium and added at concentration of 1, 10, 20 and 50 μg/ml on 12-day old cultured astrocytes. In presence of 1 and 10 μg/ml of Tiagabine, no DNA damage was observed after 48 h of treatment. A moderate DNA damage was instead observed for cells exposed to 20 μg/ml of antiepileptic drug. Finally, DNA fragmentation was more evident after treatment with 50 μg/ml of Tiagabine. We conclude that Tiagabine, at the usual recommended doses, does not appear to influence negatively the cortical rat astrocytes, inducing DNA fragmentation only at very high concentrations.
ISSN:0304-3940
1872-7972
DOI:10.1016/S0304-3940(01)01836-5