Hierarchical Internal Structures in Gelatin–Bovine Serum Albumin/β-Lactoglobulin Gels and Coacervates

• Published in: The journal of physical chemistry. B 2016-09, Vol.120 (35), p.9506-9512
• Main Authors: Pathak, Jyotsana, Rawat, Kamla, Aswal, V. K, Bohidar, H. B
• Format: Article
• Language: English
• ISSN: 1520-6106; 1520-5207
• DOI: 10.1021/acs.jpcb.6b05378

Herein, we report the comparative study of gels and complex coacervates of bovine serum albumin (BSA) and beta-lactoglobulin (β-Lg) with gelatin close to their common pI. Surface patch binding produced a range of new soft matter phases (interpolymer complexes) such as opaque coacervates (charge neutralized complexes) and transparent gels (overcharged complexes). We emphasize on the comparative study of the microstructure of coacervates and gels formed at different mixing ratios using small angle scattering (SANS) data. It was found that phase states were entirely defined by the mixing ratio r = [GB]:[β-Lg or BSA]. Thermo-viscoelastic profiles of aforesaid samples revealed a smaller storage modulus and lower melting temperature for coacervates compared to gels. Thermally activated samples generated additional phases that were also probed by SANS and rheology. Thus, it is established that intermolecular association between globular proteins and a random coil polypeptide can generate various soft matter states that may facilitate harvesting of novel biomaterials.