Yeast genes YOL002C and SUL1 are involved in neomycin resistance

In previous studies we suggested the importance of the control of plasma membrane H^sup +^-ATPase by a phosphatidylinositol-like pathway for cellular proton extrusion in Saccharomyces cerevisiae (Brandão et al. 1994; Coccetti et al. 1998). The observations that provided the model above include the i...

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Bibliographic Details
Published in:World journal of microbiology & biotechnology 2001-06, Vol.17 (4), p.399-402
Main Authors: CASTRO, I. M, CABRAL, D. B, TROPIA, M. J. M, ALMEIDA, F. M, BRANDAO, R. L
Format: Article
Language:English
Subjects:
Acidification
Biological and medical sciences
Cloning
Fundamental and applied biological sciences. Psychology
Genes. Genome
Glucose
Molecular and cellular biology
Molecular genetics
neomycin
Proteins
Protons
Saccharomyces cerevisiae
SUL1 gene
Yeast
Yeasts
YOL002C gene
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Summary:In previous studies we suggested the importance of the control of plasma membrane H^sup +^-ATPase by a phosphatidylinositol-like pathway for cellular proton extrusion in Saccharomyces cerevisiae (Brandão et al. 1994; Coccetti et al. 1998). The observations that provided the model above include the inhibition of the glucose-induced activation of the plasma membrane H^sup +^-ATPase as well as the inhibition of the glucose-induced external acidification by neomycin, a known inhibitor of the phosphatidylinositol turnover in eukaryotic cells. In this work, using two libraries, we isolated two yeast clones that were able to prevent the inhibition of glucose-induced activation of the H^sup +^-ATPase by neomycin. We show that the YOL002C gene, which encodes a protein of unknown function, and the SUL1 gene, which is a sulphate transporter belonging to the major facilitator superfamily, suppress growth inhibition by neomycin. However, they are not required for glucose-induced activation of the plasma membrane H^sup +^-ATPase. The resistance of the clones to neomycin is probably related to the level and/or activity of proteins functioning as drug extrusion pumps.[PUBLICATION ABSTRACT]
ISSN:0959-3993
1573-0972
DOI:10.1023/A:1016707627056