The Hantaan Virus Glycoprotein Precursor Is Cleaved at the Conserved Pentapeptide WAASA

The medium segment of the tripartite negative-stranded RNA genome of hantaviruses encodes for the predicted glycoprotein precursor GPC. We have demonstrated here the expression of the glycoprotein precursor of Hantaan virus following transfection of mammalian cells. The cleavage of the precursor int...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2001-10, Vol.289 (2), p.224-229
Main Authors: Löber, Christian, Anheier, Bärbel, Lindow, Steffi, Klenk, Hans-Dieter, Feldmann, Heinz
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Cercopithecus aethiops
cleavage
glycoprotein G1
glycoprotein G2
glycoprotein precursor GPC
Glycoproteins - genetics
Glycoproteins - metabolism
Hantaan virus
Hantaan virus - chemistry
Hantaan virus - metabolism
HeLa Cells
Humans
Membrane Proteins - metabolism
Molecular Sequence Data
Mutagenesis, Site-Directed
peptidase
Protein Precursors - genetics
Protein Precursors - metabolism
Protein Processing, Post-Translational
Sequence Alignment
Serine Endopeptidases - metabolism
signal peptidase complex
Structure-Activity Relationship
Transfection
Vero Cells
Viral Proteins - genetics
Viral Proteins - metabolism
WAASA motif
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Summary:The medium segment of the tripartite negative-stranded RNA genome of hantaviruses encodes for the predicted glycoprotein precursor GPC. We have demonstrated here the expression of the glycoprotein precursor of Hantaan virus following transfection of mammalian cells. The cleavage of the precursor into the glycoproteins G1 and G2 followed the rules for signal peptides and seemed to occur directly at the pentapeptide motif “WAASA.” Our data indicate that the signal peptidase complex is responsible for the proteolytic processing of the precursor GPC of Hantaan virus. The comparison of this region of the glycoprotein precursor, including the absolutely conserved WAASA motif, suggests a similar cleavage event for all hantavirus glycoproteins.
ISSN:0042-6822
1096-0341
DOI:10.1006/viro.2001.1171