Methionine hydroxy analogue enhanced fish immunity via modulation of NF-κB, TOR, MLCK, MAPKs and Nrf2 signaling in young grass carp (Ctenopharyngodon idella)

• Published in: Fish & shellfish immunology 2016-09, Vol.56, p.208-228
• Main Authors: Pan, Fei-Yu, Feng, Lin, Jiang, Wei-Dan, Jiang, Jun, Wu, Pei, Kuang, Sheng-Yao, Tang, Ling, Tang, Wu-Neng, Zhang, Yong-An, Zhou, Xiao-Qiu, Liu, Yang
• Format: Article
• Language: English
• Subjects: Aeromonas hydrophila; Aeromonas hydrophila - physiology; Animal Feed - analysis; Animals; Carps - growth & development; Ctenopharyngodon idella; Diet - veterinary; Dietary Supplements - analysis; Efficacy; Fish Diseases - genetics; Fish Diseases - immunology; Fish Diseases - microbiology; Fish Proteins - genetics; Fish Proteins - metabolism; Freshwater; Gram-Negative Bacterial Infections - genetics; Gram-Negative Bacterial Infections - immunology; Gram-Negative Bacterial Infections - microbiology; Gram-Negative Bacterial Infections - veterinary; Immune function; Immune organs; Immunity, Innate - drug effects; Methionine - administration & dosage; Methionine - analogs & derivatives; Methionine hydroxy analogue; Signal Transduction - drug effects; Structural integrity
• ISSN: 1050-4648; 1095-9947
• DOI: 10.1016/j.fsi.2016.07.020

Our study investigated the effect of dietary methionine hydroxy analogue (MHA) on growth and immunity (head kidney, spleen and skin) of young grass carp (Ctenopharyngodon idella). A total of 630 grass carp (259.70 ± 0.47 g) were fed graded levels of MHA (0, 2.4, 4.4, 6.4, 8.5 and 10.5 g/kg diet) and one dl-methionine (DLM) group (6.4 g/kg diet) for 8 weeks. At the end of the feeding trial, fish were challenged with Aeromonas hydrophila for 14 days. The results indicated that optimal MHA increased lysozyme (LZ) and acid phosphatase (ACP) activities, complement 3 (C3), C4 and immunoglobulin M (IgM) contents and up-regulated mRNA levels of liver expressed antimicrobial peptide 2, hepcidin (head kidney), β-defensin-1 in the immune organs (P