Annexin A2, autoimmunity, anxiety and depression

• Published in: Journal of autoimmunity 2016-09, Vol.73, p.92-99
• Main Authors: Weiss, R, Bitton, A, Ben Shimon, M, Elhaik Goldman, S, Nahary, L, Cooper, I, Benhar, I, Pick, C.G, Chapman, J
• Format: Article
• Language: English
• Subjects: Adjuvants, Immunologic - administration & dosage; Adrenal Cortex Hormones - blood; Allergy and Immunology; Animals; Annexin; Annexin A2 - immunology; Annexin A2 - metabolism; Antiphospholipid syndrome; Antiphospholipid Syndrome - immunology; Antiphospholipid Syndrome - psychology; Anxiety; Anxiety - blood; Anxiety - immunology; Anxiety - pathology; Autoantibodies - analysis; Autoantibodies - immunology; Autoantigens - immunology; Autoimmunity; Brain - pathology; Depression; Depression - blood; Depression - immunology; Depression - pathology; Disease Models, Animal; Enzyme-Linked Immunosorbent Assay; Female; Humans; Immunoglobulin G - analysis; Immunoglobulin G - immunology; Mice; Mice, Inbred BALB C; P11; Protein Multimerization; Psychological Tests; Recombinant Proteins - immunology; S100 Proteins - metabolism; S100A10
• ISSN: 0896-8411; 1095-9157
• DOI: 10.1016/j.jaut.2016.06.011

Abstract Objectives Antiphospholipid syndrome (APS) is associated with neurological manifestations and one of the novel autoantigens associated with this disease is Annexin A2 (ANXA2). In this work we have examined the effect of high levels of autoantibodies to ANXA2 on the brain in a mouse model. Methods Recombinant ANXA2 emulsified in adjuvant was used to immunize mice while mice immunized with adjuvant only served as controls. At peak antibody levels the animal underwent behavioral and cognitive tests and their brains were examined for ANXA2 immunoglobulin G (IgG) and expression of ANXA2 and the closely linked protein p11. Results Very high levels of anti-ANXA2 antibodies (Abs) were associated with reduced anxiety in the open field 13.14% ± 0.89% of the time in the center compared to 8.64% ± 0.91% observed in the control mice (p