A hemocyte-expressed fibrinogen-related protein gene (LvFrep) from the shrimp Litopenaeus vannamei: Expression analysis after microbial infection and during larval development

Fibrinogen-related proteins (FREPs) comprise a large family of microbial recognition proteins involved in many biological functions in both vertebrate and invertebrate animals. By taking advantage of publicly accessible databases, we have identified a FREP-like homolog in the most cultivated penaeid...

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Bibliographic Details
Published in:Fish & shellfish immunology 2016-09, Vol.56, p.123-126
Main Authors: Coelho, Jaqueline da Rosa, Barreto, Cairé, Silveira, Amanda da Silva, Vieira, Graziela Cleusa, Rosa, Rafael Diego, Perazzolo, Luciane Maria
Format: Article
Language:English
Subjects:
Animals
Crustacean immunity
Decapoda
Fibrinogen - chemistry
Fibrinogen - genetics
Fibrinogen - metabolism
Fibrinogen-related protein
Gene expression
Gene Expression Regulation
Hemocytes - metabolism
Litopenaeus vannamei
Ovum - metabolism
Pattern recognition protein/receptor
Penaeidae - classification
Penaeidae - genetics
Penaeidae - growth & development
Penaeidae - immunology
Phylogeny
Sequence Analysis, Protein
Vibrio
Vibrio - physiology
Vibrio harveyi
White spot syndrome virus
White spot syndrome virus 1 - physiology
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Summary:Fibrinogen-related proteins (FREPs) comprise a large family of microbial recognition proteins involved in many biological functions in both vertebrate and invertebrate animals. By taking advantage of publicly accessible databases, we have identified a FREP-like homolog in the most cultivated penaeid shrimp, Litopenaeus vannamei (LvFrep). The obtained sequence showed a conserved fibrinogen-related domain (FReD) and displayed significant similarities to FREP-like proteins from other invertebrates and to ficolins from crustaceans. The expression of LvFrep appeared to be limited to circulating hemocytes. Interestingly, LvFrep gene expression was induced in shrimp hemocytes only in response to a Vibrio infection but not to the White spot syndrome virus (WSSV). Moreover, LvFrep transcript levels were detected early in fertilized eggs, suggesting the participation of this immune-related gene in the antimicrobial defenses during shrimp development. •The LvFrep sequence displayed similarities to other FREP-like proteins and ficolins.•LvFrep transcripts showed to be highly expressed in circulating hemocytes.•LvFrep expression was induced in response to a Vibrio infection but not to the WSSV.•LvFrep transcripts were detected in all stages of shrimp development.
ISSN:1050-4648
1095-9947
DOI:10.1016/j.fsi.2016.06.046