Transcription of dbp from the coding region of the Bm17 gene is required for the efficient propagation of Bombyx mori nucleopolyhedrovirus

•One of the TSSs of the dbp gene is located within the Bm17 coding region.•Reduced dbp transcription results in decreased BmNPV production during infection.•The Bm17 protein has a little contribution to BmNPV pathogenicity. A Bombyx mori nucleopolyhedrovirus (BmNPV) mutant was generated, in which Bm...

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Bibliographic Details
Published in:Virus research 2016-09, Vol.223, p.57-63
Main Author: Katsuma, Susumu
Format: Article
Language:English
Subjects:
Animals
Baculoviridae
Baculovirus
Base Sequence
Bm17
BmNPV
Bombyx - virology
Bombyx mori NPV
Cell Line
DBP
Gene Expression Regulation, Viral
Larva - virology
NPV
Nucleopolyhedrovirus - physiology
Open Reading Frames
Sequence Deletion
Transcription, Genetic
Viral Proteins - chemistry
Viral Proteins - genetics
Viral Proteins - metabolism
Virus Replication
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Summary:•One of the TSSs of the dbp gene is located within the Bm17 coding region.•Reduced dbp transcription results in decreased BmNPV production during infection.•The Bm17 protein has a little contribution to BmNPV pathogenicity. A Bombyx mori nucleopolyhedrovirus (BmNPV) mutant was generated, in which Bm17 was disrupted by the insertion of a lacZ reporter cassette. This mutant (Bm17D) exhibited defective phenotypes, i.e., budded viruses (BVs) and occlusion bodies (OBs) were less produced in both B. mori cultured cells and larvae. However, a repair virus (Bm17DR), lacking endogenous Bm17 but expressing Bm17 with its endogenous promoter at a different genomic locus, did not rescue most of the defective phenotypes of Bm17D. Transcriptional units in the Bm17 region were surveyed in detail using a transcriptome map of BmNPV-infected cells. It was found that one of the transcriptional start sites (TSSs) of dbp (Bm16) is located within the Bm17 coding region and that it does not likely function in the genome of Bm17D- or Bm17DR by inserting a lacZ cassette. From the obtained results, it was shown that both dbp transcription and DBP protein expression were markedly reduced in Bm17D- or Bm17DR-infected cells. This indicates that reduced dbp transcription alone results in decreased BV and OB production during BmNPV infection.
ISSN:0168-1702
1872-7492
DOI:10.1016/j.virusres.2016.06.018