Hepatitis B virus resistance substitutions: long-term analysis by next-generation sequencing

HBV phylogenetics and resistance-associated mutations (RAMs) were surveyed by next-generation sequencing of 21 longitudinal samples from seven patients entering antiviral therapy. The virus populations were dominated by a few abundant lineages that coexisted with substantial numbers of low-frequency...

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Bibliographic Details
Published in:Archives of virology 2016-10, Vol.161 (10), p.2885-2891
Main Authors: Jones, Leandro R., Sede, Mariano, Manrique, Julieta M., Quarleri, Jorge
Format: Article
Language:English
Subjects:
Adult
Amino Acid Substitution
Antiviral Agents - therapeutic use
Biomedical and Life Sciences
Biomedicine
Brief Report
Drug resistance
Drug Resistance, Viral
Female
Genotype
Hepatitis B
Hepatitis B virus
Hepatitis B virus - classification
Hepatitis B virus - drug effects
Hepatitis B virus - genetics
Hepatitis B virus - isolation & purification
Hepatitis B, Chronic - drug therapy
Hepatitis B, Chronic - virology
High-Throughput Nucleotide Sequencing
Humans
Infectious Diseases
Interferon
Longitudinal Studies
Male
Medical Microbiology
Mutation
Patients
Phylogenetics
Phylogeny
Virology
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Summary:HBV phylogenetics and resistance-associated mutations (RAMs) were surveyed by next-generation sequencing of 21 longitudinal samples from seven patients entering antiviral therapy. The virus populations were dominated by a few abundant lineages that coexisted with substantial numbers of low-frequency variants. A few low-frequency RAMs were observed before treatment, but new ones emerged, and their frequencies increased during therapy. Together, these results support the idea that chronic HBV infection is dominated by a few virus lineages and that an accompanying plethora of diverse, low-frequency variants may function as a reservoir that potentially contribute to viral genetic plasticity, potentially affecting patient outcome.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-016-2959-8