Cryopreserved hepatic progenitor cells derived from human embryonic stem cells can arrest progression of liver fibrosis in rats

Hepatocytes generated from human embryonic stem cells (hESCs) are considered to be an excellent candidate for restoring the liver function deficiencies. We have earlier standardized a three‐step differentiation protocol to generate functional hepatocyte‐like cells (HLCs) from hESCs, which expressed...

Full description

Saved in:
Bibliographic Details
Published in:Cell biology international 2016-10, Vol.40 (10), p.1107-1115
Main Authors: Mandal, Arundhati, Raju, Sheena, Viswanathan, Chandra
Format: Article
Language:English
Subjects:
Animals
CCl4
Cell Differentiation - drug effects
Cell Line
cryopreservation
Cryopreservation - methods
Embryos
hepatocyte progenitor cells
Hepatocytes - cytology
Human Embryonic Stem Cells - cytology
Human Embryonic Stem Cells - physiology
Human Embryonic Stem Cells - transplantation
human ES cells
Humans
Liver - cytology
Liver Cirrhosis - therapy
liver fibrosis
Random Allocation
Rats
Rats, Sprague-Dawley
Rodents
Stem Cell Transplantation - methods
Stem cells
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Hepatocytes generated from human embryonic stem cells (hESCs) are considered to be an excellent candidate for restoring the liver function deficiencies. We have earlier standardized a three‐step differentiation protocol to generate functional hepatocyte‐like cells (HLCs) from hESCs, which expressed the major hepatic markers. We have also found that the HLCs remain stable and functional even after extended period of in vitro culture and cryopreservation. In the present study, we have aimed to investigate the therapeutic potential of cryopreserved‐thawed hESC‐derived hepatic progenitor cells following transplantation in carbon tetrachloride‐induced fibrotic rat livers. Significant therapeutic effects, including improved hepatic histology and normal serum biochemistry of hepatic enzymes along with increased survival rate, were observed in the cell transplanted rats. This result is an encouraging indication to develop methods for clinical application of hESC‐derived hepatic lineage cells.
ISSN:1065-6995
1095-8355
DOI:10.1002/cbin.10649