Triggered isothermal PCR by denaturation bubble-mediated strand exchange amplification

Here, we introduced the concept of strand exchange amplification (SEA) mediated by denaturation bubbles. Similar to traditional PCR, it only employed a DNA polymerase and a pair of common primers to realize a three-step cycle process, but the entire SEA reaction was performed at a single temperature...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2016-01, Vol.52 (77), p.11551-11554
Main Authors: Shi, Chao, Shang, Fanjin, Zhou, Meiling, Zhang, Pansong, Wang, Yifan, Ma, Cuiping
Format: Article
Language:English
Subjects:
Amplification
Bubbles
Denaturation
DNA polymerase
Exchange
Strands
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Summary:Here, we introduced the concept of strand exchange amplification (SEA) mediated by denaturation bubbles. Similar to traditional PCR, it only employed a DNA polymerase and a pair of common primers to realize a three-step cycle process, but the entire SEA reaction was performed at a single temperature. Here, we introduced the concept of strand exchange amplification (SEA) mediated by denaturation bubbles.
ISSN:1359-7345
1364-548X
DOI:10.1039/c6cc05906f