Induction of Intestinal Rotavirus-Specific Antibodies in Respiratory, but Not Gut, Lymphoid Tissues Following Mucosal Immunization of Mice with Inactivated Rotavirus

Intranasal (i.n.), but not oral, immunization of mice with inactivated rotavirus induces protection against challenge. To understand the mechanisms by which i.n. immunization with inactivated rotavirus evokes protective immunity, we examined the site of rotavirus-specific B cell activation and the o...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2001-12, Vol.291 (2), p.235-240
Main Authors: Coffin, Susan E., Clark, Stephanie L.
Format: Article
Language:English
Subjects:
Administration, Intranasal
Animals
Antibodies, Viral - biosynthesis
Disease Models, Animal
Female
IgA
inactivated virus
Intestine, Small - immunology
Intestine, Small - virology
Lymphoid Tissue - immunology
Mice
Mice, Inbred BALB C
mouse
Nasal Mucosa - immunology
protection
Respiratory System - immunology
respiratory tract
Rotavirus
Rotavirus - immunology
Rotavirus Infections - prevention & control
Vaccination
vaccine
Vaccines, Inactivated
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Summary:Intranasal (i.n.), but not oral, immunization of mice with inactivated rotavirus induces protection against challenge. To understand the mechanisms by which i.n. immunization with inactivated rotavirus evokes protective immunity, we examined the site of rotavirus-specific B cell activation and the origins of intestinal IgA-secreting B cells following i.n. inoculation of mice with inactivated rhesus rotavirus. We found that (1) i.n., but not oral, inoculation induced partial protection after challenge; (2) i.n., but not oral, inoculation induced production of rotavirus-specific IgM, IgA, and IgG by intestinal lymphoid tissues; and (3) after i.n. inoculation, nasal-associated lymphoid tissues (NALT) and bronchial lymph nodes (BLN) were the sites of initial production of rotavirus-specific antibodies. These studies indicate that after inoculation with inactivated rotavirus, virus-specific effector B cells may be more easily activated in respiratory, compared to intestinal, lymphoid tissues. Additional studies are needed to determine if these observations are due to fundamental differences in the microenvironment of NALT and BLN compared to Peyer's patches or are a function of the anatomic differences between the respiratory and the gastrointestinal tracts.
ISSN:0042-6822
1096-0341
DOI:10.1006/viro.2001.1180