One-step transformation of two Andean potato cultivars (Solanum tuberosum L. subsp. andigena)

• Published in: Plant cell reports 2001-10, Vol.20 (7), p.637-641
• Main Authors: TRUJILLO, C, RODRIGUEZ-ARANGO, E, JARAMILLO, S, HOYOS, R, ORDUZ, S, ARANGO, R
• Format: Article
• Language: English
• Subjects: Acetic acid; Agrobacterium tumefaciens; Agronomy. Soil science and plant productions; Biological and medical sciences; Biotechnology; Cultivars; Explants; Fundamental and applied biological sciences. Psychology; Genetic engineering; Genetic engineering applications; Genetic technics; Genetic transformation; Genetics and breeding of economic plants; GUS gene; Indoleacetic acid; Kanamycin; Methods. Procedures. Technologies; nptII gene; Plant breeding: fundamental aspects and methodology; Plantlets; Polymerase chain reaction; Potatoes; Regeneration; Solanum tuberosum; Southern blotting; Transgenic animals and transgenic plants; Transgenic plants; Zeatin
• ISSN: 0721-7714; 1432-203X
• DOI: 10.1007/s002990100381

Most Agrobacterium-mediated transformation procedures described in the literature have proven to be inefficient for transformation of Andean potato cultivars. We describe here a more successful method for transformation of two of these cultivars, Diacol Capiro (DC) and Parda Pastusa (PP). Leaf explants were transformed using vector pBI121 carrying the β-glucuronidase and kanamycin resistance genes. Calli and shoot formation occurred after 5–8 weeks on a selection medium containing 3 mg/l zeatin and 1 mg/l indole acetic acid. The shoot regeneration frequency was 28% and 34% for DC and PP, respectively. Of the kanamycin-resistant plantlets obtained, 51% of those of DC and 13% of those of PP were confirmed to be transformants by histochemical assays, polymerase chain reaction analyses and Southern blotting techniques.