Activation of hypoxia inducible factor-1α in gastric mucosa in response to ethanol injury: A trigger for angiogenesis?

Gastric mucosal injury triggers angiogenesis and activation of VEGF expression, but the mechanism(s) of VEGF gene activation are not known. In some tissues (e.g. myocardium), hypoxia triggers activation of hypoxia-inducible factor-1α (HIF-1α), a transcription factor known to activate VEGF gene expre...

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Bibliographic Details
Published in:Life sciences (1973) 2001-11, Vol.69 (25), p.3035-3044
Main Authors: Szabo, Imre L., Kawanaka, Hirofumi, Jones, Michael K., Pai, Rama, Soreghan, Brian A., Baatar, Dolgor, Husain, Syeda S., Tarnawski, Andrzej S.
Format: Article
Language:English
Subjects:
Ethanol-injury
HIF-1α
Hypoxia
hypoxia-inducible factor-1^a
VEGF
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Summary:Gastric mucosal injury triggers angiogenesis and activation of VEGF expression, but the mechanism(s) of VEGF gene activation are not known. In some tissues (e.g. myocardium), hypoxia triggers activation of hypoxia-inducible factor-1α (HIF-1α), a transcription factor known to activate VEGF gene expression. This study was aimed to determine whether hypoxia and/or alcohol injury may induces HIF-1α in gastric mucosa. Normal rat gastric tissue was incubated in organ culture under either hypoxic or normoxic conditions for 6hrs. Rats received, intragastrically, either saline or alcohol and gastric mucosa bordering necrosis was obtained at 1–24hrs. HIF-1α mRNA and protein were determined by RT-PCR and Western-blot analysis. HIF-1α and VEGF proteins were localized by immunostaining. Incubation of normal gastric mucosa under hypoxia caused a significant elevation of HIF-1α mRNA (20±2%, p
ISSN:0024-3205
1879-0631
DOI:10.1016/S0024-3205(01)01410-2