Association between BMI and gene expression of anti-Müllerian hormone and androgen receptor in human granulosa cells in women with and without polycystic ovary syndrome

• Published in: Clinical endocrinology (Oxford) 2016-10, Vol.85 (4), p.590-595
• Main Authors: Nouri, Mohammad, Aghadavod, Esmat, Khani, Sajad, Jamilian, Mehri, Amiri Siavashani, Mehrnush, Ahmadi, Shahnaz, Asemi, Zatollah
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Anti-Mullerian Hormone - genetics; Anti-Mullerian Hormone - metabolism; Body Mass Index; Case-Control Studies; Cross-Sectional Studies; Female; Gene Expression; Granulosa Cells - metabolism; Humans; Polycystic Ovary Syndrome - metabolism; Polycystic Ovary Syndrome - pathology; Real-Time Polymerase Chain Reaction; Receptors, Androgen - genetics; Receptors, Androgen - metabolism; Young Adult
• ISSN: 0300-0664; 1365-2265
• DOI: 10.1111/cen.13098

Summary Background Anti‐Müllerian hormone (AMH) is one of the most reliable markers of ovarian reserve. There is evidence which suggests that BMI may be associated with gene expression of AMH, AMH type II receptor (AMHR‐II) and androgen receptor (AR) in human granulosa cells (GC) in women with and without polycystic ovary syndrome (PCOS). Objective To investigate the association between BMI and gene expression of AMH, AMHR‐II and AR in human GC in women with and without PCOS. Design, Patients and Measurements In a cross‐sectional study, hormonal profiles were measured among 38 patients with PCOS and 38 subjects without PCOS aged 18–40. AMH, AMHR‐II and AR mRNA levels were quantified in cumulus GC. Pearson correlation and multiple linear regressions were used to assess the relationships. Results Quantitative RT‐PCR demonstrated that AMH and AMHR‐II expression were negatively correlated with BMI (r = −0·39, P