Metabolism of methiocarb and carbaryl by rat and human livers and plasma, and effect on their PXR, CAR and PPARα activities

• Published in: Journal of toxicological sciences 2016/10/01, Vol.41(5), pp.677-691
• Main Authors: Fujino, Chieri, Tamura, Yuki, Tange, Satoko, Nakajima, Hiroyuki, Sanoh, Seigo, Watanabe, Yoko, Uramaru, Naoto, Kojima, Hiroyuki, Yoshinari, Kouichi, Ohta, Shigeru, Kitamura, Shigeyuki
• Format: Article
• Language: English
• Subjects: Animals; Biotransformation; Carbamates (tradename); Carbaryl; Carbaryl - blood; Carbaryl - metabolism; Carbaryl - toxicity; Cercopithecus aethiops; Cholinesterase Inhibitors - metabolism; Cholinesterase Inhibitors - toxicity; COS Cells; CYP1A2 protein; Cytochrome; Cytochrome P-450 CYP1A2 - metabolism; Cytochrome P-450 CYP2C19 - metabolism; Cytochrome P450; Cytochromes P450; Cytosol; Dimethylaniline monooxygenase (N-oxide-forming); Fish oils; Flavin; Humans; Hydrolysis; Liver; Liver - metabolism; Male; Metabolic pathways; Metabolism; Metabolites; Methiocarb; Methiocarb - blood; Methiocarb - metabolism; Methiocarb - toxicity; Microsomes; Microsomes, Liver - metabolism; NADP; Naphthol; Oxidation; Oxidation-Reduction; Oxidative metabolism; PPAR alpha - agonists; PPAR alpha - genetics; PPAR alpha - metabolism; PXR; Rats, Sprague-Dawley; Receptors, Cytoplasmic and Nuclear - agonists; Receptors, Cytoplasmic and Nuclear - genetics; Receptors, Cytoplasmic and Nuclear - metabolism; Receptors, Steroid - agonists; Receptors, Steroid - genetics; Receptors, Steroid - metabolism; Reductive metabolism; Sulfoxides; Transfection
• ISSN: 0388-1350; 1880-3989
• DOI: 10.2131/jts.41.677

The oxidative, reductive, and hydrolytic metabolism of methiocarb and the hydrolytic metabolism of carbaryl by liver microsomes and plasma of rats or humans were examined. The effects of the metabolism of methiocarb and carbaryl on their nuclear receptor activities were also examined. When methiocarb was incubated with rat liver microsomes in the presence of NADPH, methiocarb sulfoxide, and a novel metabolite, methiocarb sulfone were detected. Methiocarb sulfoxide was oxidized to the sulfone by liver microsomes and reduced back to methiocarb by liver cytosol. Thus, the interconversion between methiocarb and the sulfoxide was found to be a new metabolic pathway for methiocarb by liver microsomes. The product of methiocarb hydrolysis, which is methylthio-3,5-xylenol (MX), was also oxidized to sulfoxide form by rat liver microsomes. The oxidations were catalyzed by human flavin-containing monooxygenase isoform (FMO1). CYP2C19, which is a human cytochrome P450 (CYP) isoform, catalyzed the sulfoxidations of methiocarb and MX, while CYP1A2 also exhibited oxidase activity toward MX. Methiocarb and carbaryl were not enzymatically hydrolyzed by the liver microsomes, but they were mainly hydrolyzed by plasma and albumin to MX and 1-naphthol, respectively. Both methiocarb and carbaryl exhibited PXR and PPARα agonistic activities; however, methiocarb sulfoxide and sulfone showed markedly reduced activities. In fact, when methiocarb was incubated with liver microsomes, the receptor activities were decreased. In contrast, MX and 1-naphthol showed nuclear receptor activities equivalent to those of their parent carbamates. Thus, the hydrolysis of methiocarb and carbaryl and the oxidation of methiocarb markedly modified their nuclear receptor activities.