Rapid decrease of RNA level of a novel mouse mitochondria solute carrier protein (Mscp) gene at 4–5 weeks of age

We cloned a novel mouse gene that encodes a protein with homology to the mitochondria solute carrier proteins (Mscp). The major full-length Mscp transcript contains 4112 bp of cDNA and a deduced protein of 338 amino acids. The Mscp protein shares 50%, 40%, and 39% sequence identity with the C. elega...

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Bibliographic Details
Published in:Mammalian genome 2001-11, Vol.12 (11), p.830-836
Main Authors: Li, Quan-Zhen, Eckenrode, Sarah, Ruan, Qing-Guo, Wang, Cong-Yi, Shi, Jing-Da, McIndoe, Richard A, She, Jin-Xiong
Format: Article
Language:English
Subjects:
Amino Acid Sequence
amino acids
Animals
Base Sequence
Blotting, Northern
Carrier Proteins - genetics
cDNA microarrays
Cloning, Molecular
complementary DNA
DNA, Complementary - chemistry
DNA, Complementary - genetics
energy transfer
Female
Gene Expression
Gene Expression Regulation, Developmental
genes
messenger RNA
Mice
Mice, Inbred C57BL
Mice, Inbred NOD
microarray technology
Mitochondria
mitochondria solute carrier protein
Molecular Sequence Data
Mscp gene
Proteins
RNA - genetics
RNA - metabolism
Rodents
Sequence Alignment
sequence analysis
Sequence Analysis, DNA
Sequence Homology, Amino Acid
solutes
spleen
Time Factors
transport proteins
yeasts
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Summary:We cloned a novel mouse gene that encodes a protein with homology to the mitochondria solute carrier proteins (Mscp). The major full-length Mscp transcript contains 4112 bp of cDNA and a deduced protein of 338 amino acids. The Mscp protein shares 50%, 40%, and 39% sequence identity with the C. elegans hypothetical protein T26089 and the yeast mitochondria carrier proteins MRS3 and MRS4, respectively. It also showed homology with the uncoupling proteins (UCP1, UCP2, and UCP3; 22%, 24%, and 29% identity, respectively). The protein has six transmembrane domains and three mitochondria energy-transfer protein signature motifs, which are conserved among all the members of mitochondria carrier protein family. Northern analysis indicated that the Mscp gene is highly expressed in the spleen. Using cDNA microarray and Northern analysis, we have shown a significant decrease of the splenic Mscp mRNA levels around 4–5 weeks of age in several mouse strains including C57BL/6J, nonobese diabetic (NOD), and several NOD-congenic mice. These results suggest that the Mscp gene is decreased during splenic lymphocyte maturation in these mice.
ISSN:0938-8990
1432-1777
DOI:10.1007/s00335001-2075-1