Binding sites for interaction of peroxiredoxin 6 with surfactant protein A

• Published in: Biochimica et biophysica acta 2016-04, Vol.1864 (4), p.419-425
• Main Authors: Krishnaiah, Saikumari Y., Dodia, Chandra, Sorokina, Elena M., Li, Haitao, Feinstein, Sheldon I., Fisher, Aron B.
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Binding Sites; Circular Dichroism; Isothermal titration calorimetry; Lung lamellar bodies; Lung surfactant; Molecular Sequence Data; Peroxiredoxin VI - chemistry; Phospholipase A2; Phospholipases A2 - metabolism; Pulmonary Surfactant-Associated Protein A - chemistry
• ISSN: 1570-9639; 0006-3002; 1878-1454
• DOI: 10.1016/j.bbapap.2015.12.009

Peroxiredoxin 6 (Prdx6) is a bifunctional enzyme with peroxidase and phospholipase A2 (PLA2) activities. This protein participates in the degradation and remodeling of internalized dipalmitoylphosphatidylcholine (DPPC), the major phospholipid component of lung surfactant. We have shown previously that the PLA2 activity of Prdx6 is inhibited by the lung surfactant-associated protein called surfactant protein A (SP-A) through direct protein-protein interaction. Docking of SPA and Prdx6 was modeled using the ZDOCK (zlab.bu.edu) program in order to predict molecular sites for binding of the two proteins. The predicted peptide sequences were evaluated for binding to the opposite protein using isothermal titration calorimetry and circular dichroism measurement followed by determination of the effect of the SP-A peptide on the PLA2 activity of Prdx6. The sequences 195EEEAKKLFPK204.in the Prdx6 helix and 83DEELQTELYEIKHQIL99 in SP-A were identified as the sites for hydrophobic interaction and H+-bonding between the 2 proteins. Treatment of mouse endothelial cells with the SP-A peptide inhibited their recovery from lipid peroxidation associated with oxidative stress indicating inhibition of Prdx6 activity by the peptide in the intact cell. •Peptide sites for interaction of surfactant protein A (SP-A) and peroxiredoxin 6 (Prdx6) have been identified•SP-A peptide destabilized the secondary structure of Prdx6 and inhibited its PLA2 activity•Recovery of endothelial cells from peroxidative stress was markedly delayed by inhibition of PLA2 activity